Connect with us

Heartland

Therapeutic Use of Δ9-THC and Cannabidiol: Evaluation of a New Extraction Procedure for the Preparation of Cannabis-based Olive Oil

CONCLUSION: The developed method was simple and fast. The extraction procedure proved to be highly reproducible and applicable routinely to cannabis preparations.

Republished by Plato

Published

on

Background: Since 2013 Cannabis-based preparations, containing the two main cannabinoids of interest, Δ9-tetrahydrocannabinol (THC), and cannabidiol (CBD), can be used for therapeutic purposes, such as palliative care, neurodegenerative disorder treatment and other therapies. The preparations may consist of a drug partition in sachets, capsules or through the extraction in certified olive oil.

Objective: The aims of the study were: a) to develop and validate a new liquid chromatographictandem mass spectrometric (LC-MS/MS) method for the identification and quantification of THC and CBD in olive oil; b) to evaluate the extraction efficiency and reproducibility of a new commercial extractor on the market.

Methods: The olive oil was simply diluted three consecutive times, using organic solvents with increasing polarity index (n-hexane → isopropanol → methanol). The sample was then directly injected into LC-MS/MS system, operating in Multiple Reaction Monitoring Mode, in positive polarization. The method was then fully validated.

Results: The method assessed to be linear over the range 0.1-10 ng/µL for both THC and CBD. Imprecision and accuracy were within 12.2% and 16.9% respectively; matrix effects proved to be negligible; THC concentration in oil is stable up to two months at room temperature, whenever kept in the dark. CBD provided a degradation of 30% within ten weeks. The method was then applied to olive oil after sample preparation, in order to evaluate the efficiency of extraction of a new generation instrument. Temperature of extraction is the most relevant factor to be optimized. Indeed, a difference of 2°C (from 94.5°C to 96.5°C, the highest temperature reached in the experiments) of the heating phase, increases the percentage of extraction from 54.2% to 64.0% for THC and from 58.2% to 67.0% for CBD. The amount of THC acid and CBD acid that are decarboxylated during the procedure must be check out in the future.

Conclusion: The developed method was simple and fast. The extraction procedure proved to be highly reproducible and applicable routinely to cannabis preparations.

Keywords: LC-MS/MS; cannabidiol; cannabis preparations; olive oil; therapeutic cannabinoids; Δ9-tetrahydrocannabinol.

Source: https://pubmed.ncbi.nlm.nih.gov/29189144/?utm_source=no_user_agent&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1TUbR4JfRTv1rbahLd1HaiF0w5ryO5ocWB3_xk3Prq78L0AEhI&fc=20200804222312&ff=20200817013501&v=2.11.5

Heartland

Republished by Plato

Published

on

Coinsmart. Beste Bitcoin-Börse in Europa
Source:

Continue Reading

Heartland

Republished by Plato

Published

on

Coinsmart. Beste Bitcoin-Börse in Europa
Source:

Continue Reading

Heartland

Republished by Plato

Published

on

Coinsmart. Beste Bitcoin-Börse in Europa
Source:

Continue Reading
Uncategorized4 days ago

Halo Collective Announces New Appointment to Board of Directors and Completes Securities Issuance to Independent Consultants, Related Parties, and Suppliers

Metal4 days ago

Registration open for IHEA annual meeting

Heartland2 days ago

Real Brands Inc. CEO Discusses Corporate Goals and Resolve in Audio Interview with …

Heartland4 days ago

Asia Pacific IoT command centre to be established in Adelaide

Heartland2 days ago

Harvard Study Finds Cannabis Effective for Chronic Pain

Heartland1 day ago

First time with WNC or any Indoor CBD in general.

Heartland4 days ago

does cbd help with dizziness?

Heartland1 day ago

Any sales for goin on ?

Heartland2 days ago

I just bought this brand that I have never seen before. Has anyone ever heard of it?

Heartland3 days ago

Are Delta-8 THC Syringes the Most Practical Way to Medicate?

Heartland21 hours ago

At Virtual Forum, Wisconsin Gov. Tony Evers Calls on Lawmakers to Legalize Cannabis

Heartland5 days ago

Heartland5 days ago

Heartland5 days ago

Heartland4 days ago

Heartland4 days ago

Heartland4 days ago

Heartland10 hours ago

The future of the cannabis industry and sports

Heartland4 days ago

Auckland CBD shop among newest locations of interest for Covid-positive border worker

Heartland4 days ago

Heartland4 days ago

Heartland3 days ago

Heartland2 days ago

Heartland4 days ago

Heartland4 days ago

Heartland4 days ago

Northeast Regulators Talk Cannabis, Real Estate, and Regional Collaboration

Heartland5 days ago

Do you have a favorite CBD product?

News5 days ago

Get Ready for Some Volatility

Heartland4 days ago

Are there usually deals on 4/20? Especially at WNC.

Heartland4 days ago

What’s the best flower in EU? (<0.2% THC)

Trending

A Cloud Nine Capital Entity Copyright © 2020 – All Rights Reserved Proudly Made in America