Connect with us

Heartland

The Cannabinoids Δ(8)THC, CBD, and HU-308 Act via Distinct Receptors to Reduce Corneal Pain and Inflammation

Background and Purpose: Corneal injury can result in dysfunction of corneal nociceptive signaling and corneal sensitization. Activation of the endocannabinoid system has been reported to be analgesic and anti-inflammatory. The purpose of this research was to investigate the antinociceptive and anti-inflammatory effects of cannabinoids with reported actions at cannabinoid 1 (CB(1)R) and cannabinoid 2 (CB(2)R) receptors and/or noncannabinoid receptors in an experimental model of corneal…

Republished by Plato

Published

on

Background and Purpose: Corneal injury can result in dysfunction of corneal nociceptive signaling and corneal sensitization. Activation of the endocannabinoid system has been reported to be analgesic and anti-inflammatory. The purpose of this research was to investigate the antinociceptive and anti-inflammatory effects of cannabinoids with reported actions at cannabinoid 1 (CB1R) and cannabinoid 2 (CB2R) receptors and/or noncannabinoid receptors in an experimental model of corneal hyperalgesia. Methods: Corneal hyperalgesia (increased pain response) was generated using chemical cauterization of the corneal epithelium in wild-type (WT) and CB2R knockout (CB2R-/-) mice. Cauterized eyes were treated topically with the phytocannabinoids Δ8-tetrahydrocannabinol (Δ8THC) or cannabidiol (CBD), or the CBD derivative HU-308, in the presence or absence of the CB1R antagonist AM251 (2.0 mg/kg i.p.), or the 5-HT1A receptor antagonist WAY100635 (1 mg/kg i.p.). Behavioral pain responses to a topical capsaicin challenge at 6 h postinjury were quantified from video recordings. Mice were euthanized at 6 and 12 h postcorneal injury for immunohistochemical analysis to quantify corneal neutrophil infiltration. Results: Corneal cauterization resulted in hyperalgesia to capsaicin at 6 h postinjury compared to sham control eyes. Neutrophil infiltration, indicative of inflammation, was apparent at 6 and 12 h postinjury in WT mice. Application of Δ8THC, CBD, and HU-308 reduced the pain score and neutrophil infiltration in WT mice. The antinociceptive and anti-inflammatory actions of Δ8THC, but not CBD, were blocked by the CB1R antagonist AM251, but were still apparent, for both cannabinoids, in CB2R-/- mice. However, the antinociceptive and anti-inflammatory actions of HU-308 were absent in the CB2R-/- mice. The antinociceptive and anti-inflammatory effects of CBD were blocked by the 5-HT1A antagonist WAY100635. Conclusion: Topical cannabinoids reduce corneal hyperalgesia and inflammation. The antinociceptive and anti-inflammatory effects of Δ8THC are mediated primarily via CB1R, whereas that of the cannabinoids CBD and HU-308, involve activation of 5-HT1A receptors and CB2Rs, respectively. Cannabinoids could be a novel clinical therapy for corneal pain and inflammation resulting from ocular surface injury.

Keywords: cannabinoids; cornea; hyperalgesia; inflammation; pain.

Source: https://pubmed.ncbi.nlm.nih.gov/29450258/?utm_source=no_user_agent&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1TQbL92JdzYTve0m3OsSoggMby2JyZDVBLRHiDMYZkT1hs6YNQ&fc=20200804223051&ff=20200817123621&v=2.11.5

Heartland

Republished by Plato

Published

on

Source:

Continue Reading

Heartland

Republished by Plato

Published

on

Source:

Continue Reading

Heartland

Republished by Plato

Published

on

Source:

Continue Reading
Heartland20 hours ago

CBD Vape Oil Market 2021: Global Trends, Business Overview, Challenges, Opportunities …

Heartland5 days ago

Heartland5 days ago

What are Acidic Cannabinoids, and How Do They Work?

Heartland4 days ago

Uncategorized4 days ago

What CBD Oil is Right For Me?

Heartland4 days ago

Heartland3 days ago

Heartland4 days ago

Registration opens for consumable hemp manufacturers and retailers

Heartland5 days ago

It's all about the vibe

Heartland4 days ago

CBD Oil for Beginners

Heartland4 days ago

CBP Can't Block Parts Imports, Hemp Machinery Co. Says

Heartland3 days ago

8 of the best cannabis gummies to try right now

Heartland5 days ago

website for flower

Heartland5 days ago

Heartland4 days ago

Interactions between CBD and hormonal contraception

Metal4 days ago

Pakistani CRC and HDG producers raise prices on higher input costs

Heartland4 days ago

CBD Skincare Products Are Popping Up Everywhere — But Do They Actually Work?

Heartland3 days ago

Heartland3 days ago

Heartland2 days ago

National Hemp Association Awards First Recipients of Social Equity Conscious Business (SECB …

Heartland5 days ago

Nebraska Ag Expo kicks off at Lancaster Event Center

Heartland5 days ago

Superior Vibes CBD Oil

Material4 days ago

IMC microstructure modification and mechanical reinforcement of Sn–Ag–Cu/Cu microelectronic joints through an advanced surface finish technique

Heartland4 days ago

Sunnyvale Labs CBD Gummies — Instant Pain Relief Hemp Gummies!

Heartland4 days ago

Material4 days ago

A new approach of predicting dynamic recrystallization using directly a flow stress model and its application to medium Mn steel

Heartland4 days ago

Hemp Is Creating an EcoRevolution

Heartland4 days ago

CBD to combat physcoactive properties in THC

Heartland2 days ago

Industrial Hemp in Automotive Market Estimated Forecast Analysis 2021-2030

Heartland5 days ago

For Comedian Alonzo Bodden, Home Is Where The Stage Is

Trending

A Cloud Nine Capital Entity Copyright © 2020 – All Rights Reserved Proudly Made in America