Heartland

THC exposure of human iPSC neurons impacts genes associated with neuropsychiatric disorders

Published

3 years ago

April 26, 2018

There is a strong association between cannabis use and schizophrenia but the underlying cellular links are poorly understood. Neurons derived from human-induced pluripotent stem cells (hiPSCs) offer a platform for investigating both baseline and dynamic changes in human neural cells. Here, we exposed neurons derived from hiPSCs to Δ⁹-tetrahydrocannabinol (THC), and identified diagnosis-specific differences not detectable in vehicle-controls. RNA transcriptomic analyses revealed that THC administration, either by acute or chronic exposure, dampened the neuronal transcriptional response following potassium chloride (KCl)-induced neuronal depolarization. THC-treated neurons displayed significant synaptic, mitochondrial, and glutamate signaling alterations that may underlie their failure to activate appropriately; this blunted response resembles effects previously observed in schizophrenia hiPSC- derived neurons. Furthermore, we show a significant alteration in THC-related genes associated with autism and intellectual disability, suggesting shared molecular pathways perturbed in neuropsychiatric disorders that are exacerbated by THC.

Source: https://pubmed.ncbi.nlm.nih.gov/29691375/?utm_source=no_user_agent&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1z7zlNEMzQex5jriqkhCblNtckSEpT9xYlxxeymU_73UTETESY&fc=20200804213258&ff=20200810102726&v=2.11.5

Heartland

My experience with CBD

Published

3 hours ago

October 31, 2020

Republished by Plato

I will not spend an hour summarizing my mental health. I will just say that for years I have been plagued by depression, anxiety, antisocial feelings, and over the past year or so a feeling of brain fog. I was diagnosed with ADD as a child and they decided I didn’t have it 2 years later but I know I likely still do. I decided to finally give CBD from the local Rite aid a try. After 4 days I can say that the brain fog is gone. A week ago I couldn’t even explain the feeling that plagued me. I still don’t feel anything close to a normal person but I can say that I have a better ability to process my brain that I haven’t felt in years. I reccomend anyone who is interested give CBD a try. I only hope the clowns in our country can get past the marijuana BS that they try to strong along and connect to CBD.

Source: https://www.reddit.com/r/CBD/comments/jlasaf/my_experience_with_cbd/

Heartland

Anyone tried 10DollarCartridge CBD carts?

Published

4 hours ago

October 31, 2020

Republished by Plato

Visit our community site for vetted suppliers at http://theCBD.place. It’s time that this subject was given more internet exposure. We are here to discuss topics related to medical marijuana and our experiences using CBD. Please do not assume that anyone here is a medical professional.

Source: https://www.reddit.com/r/CBD/comments/jl95ga/anyone_tried_10dollarcartridge_cbd_carts/

Heartland

Anxiety and ADD?

Published

6 hours ago

October 30, 2020

Republished by Plato

I went to a doctor to try and sort out my anxiety issues, they are becoming a bit much to self manage. They put me on Prozac and it was a complete nightmare, the side effects were horrible, not to mention all of the unspoken rules that came along with it, no drinking, no ibuprofen, and countless others.

I am a regular THC user and that seems to help control my anxiety in the evenings but I obviously do not use at work which is where the problem lies.

I have issues with general anxiety, social anxiety, and add. Can CBD help me? How do I know how much to take, and do I have to keep taking it as in does it wear off ever couple hours? I dont know anything about this stuff but I know that Prozac is NOT for me!!!

Source: https://www.reddit.com/r/CBD/comments/jl7gfk/anxiety_and_add/