Heartland

Textile Hemp vs. Salinity: Insights from a Targeted Gene Expression Analysis

Published

3 years ago

September 29, 2017

Soil salinity is a serious threat to agriculture, because it compromises biomass production and plant productivity, by negatively affecting the vegetative growth and development of plants. Fiber crops like textile hemp (Cannabis sativa L.) are important natural resources that provide, sustainably, both cellulosic and woody fibers for industry. In this work, the response to salinity (200 mM NaCl) of a fiber variety of hemp (Santhica 27) was studied using quantitative real-time PCR. The responses of plantlets aged 15 days were analyzed by microscopy and by measuring the changes in expression of cell wall-related genes, as well as in the general response to exogenous constraints. The results presented here show that a different response is present in the hemp hypocotyls and leaves. In the leaves, genes coding for heat shock proteins were significantly upregulated, together with a phytohormone-related transcript (ethylene-responsive factor 1 ERF1) and genes involved in secondary cell wall biosynthesis (cellulose synthase CesA4, fasciclin-like arabinogalactan proteins FLA10 and FLA8). Moreover, a tendency towards upregulation was also observed in the leaves for genes involved in lignification (4CL, CAD, PAL); a finding that suggests growth arrest. In the hypocotyl, the genes involved in lignification did not show changes in expression, while a gene related to expansion (expansin EXPA8), as well as transcripts coding for calcium-dependent lipid-binding family proteins (CALB), were upregulated. Microscopic analyses on the hypocotyl cross sections revealed changes in the vascular tissues of salt-exposed plantlets, where the lumen of xylem vessels was reduced. The gene expression results show that a different response is present in the hemp hypocotyls and leaves. The data presented contribute to our understanding of the regulatory gene network in response to salinity in different tissues of an important fiber crop.

Keywords: bast fibers; cell wall; gene expression; hemp; salinity.

Source: https://pubmed.ncbi.nlm.nih.gov/28954403/?utm_source=no_user_agent&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1XAOX_jnjTyCUo06Xbq0lslwi760CIxcWfQx0n9x5BPH5yV1sN&fc=20200804212910&ff=20200811100505&v=2.11.5

Related Topics:analysis cannabis Data development expansion growth heartland hemp industry Regulatory

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Heartland

Industrial hemp seminar held in Chamberlain

Published

1 hour ago

October 24, 2020

Republished by Plato

CHAMBERLAIN, S.D. (KELO) — About a dozen people turned out Saturday for a meeting on industrial hemp in South Dakota.

The event was organized by producers and business people from Nebraska, where some farmers have been producing low-THC hemp.

South Dakota is getting ready to dip its toe into the hemp field. State legislators passed a law this year legalizing hemp with no more than 0.3% THC. The U.S. Department of Agriculture approved South Dakota’s plan October 15. A state rules hearing is set for November 13.

South Dakota Senate Democratic leader Troy Heinert of Mission started working on industrial hemp years ago. He sponsored a bill in 2016 that the House of Representatives passed but died after a Senate committee hearing.

South Dakota lawmakers passed a bill last year but Governor Kristi Noem vetoed it. This year she agreed to sign it.

“I’m sorry we are not as far along as we should be, because we’ve been talking about this for a long time,” Heinert said.

What made the difference, he said, was that several tribal governments in South Dakota received USDA approval for their plans. They needed to be able to transport hemp outside their reservations.

“Tribes were now allowed to grow hemp,” said Heinert, a member of the Rosebud Sioux Tribe. “That changed things.”

He noted that South Dakota is only state in the nation where ingestion and possession of marijuana are felony crimes.

Another speaker Saturday was Katie Sieverding. She is executive director for the South Dakota Industrial Hemp Association. Sieverding said the federal government wants modifications to South Dakota’s new law. She said the Noem administration is expected to bring a lengthy bill for the 2021 legislative session.

Ken Meyer, from Winfred-based A.H. Meyer and Sons, said the company plans to use CO2 to produce CBD oil after it receives a South Dakota hemp-processor license. The company currently specializes in rendering and selling beeswax to honey producers.

Adam Brewer founded CBD Remedies in Lincoln, Nebraska. The company focuses on retail sales and also manufactures products. Brewer said packaging that includes THC data is important for consumer confidence.

Derek Kats, who started Hemp Consultants in Lincoln, Nebraska, three years ago, said Nebraska is “very behind” in its processing for industrial hemp. “The cultivation side is key, but unless all these other pieces are put in place, the cultivation bottle-necks,” Kats said.

Source: https://www.google.com/url?rct=j&sa=t&url=https://www.keloland.com/news/capitol-news-bureau/industrial-hemp-seminar-held-in-chamberlain/&ct=ga&cd=CAIyGjYxZmRhODRmNjY4MjhhNTU6Y29tOmVuOlVT&usg=AFQjCNGa2DXqilXAUH6NHJMLp2Owxao7OQ

Heartland

Tryplainjane 1000 mg CBD Full Spectrum Oil $29 Legit or Scam?

Published

1 hour ago

October 24, 2020

Republished by Plato

I have no info on this brand. Tbh that is the price is closer to what we all should be paying for cbd. Most companies use $10 worth of cbd isolate, slap a fancy label, and maybe a dash of full spectrum and charge $100 for it. I started making my own cbd/cbg/cbn oils. Veteran grown has great prices on cbd isolate. Industrial hemp farms has the best prices on cbd cbg and cbn. 1 gram of isolate is 990 mg of cbd. Put it in warm coconut oil. Stir until dissolved and poof you have cbd oil. ✌🏻💚🌳

Edit: this is why I layer my cannaboids https://www.chiroeco.com/entourage-cbd/amp/

Source: https://www.reddit.com/r/CBD/comments/jhfjwh/tryplainjane_1000_mg_cbd_full_spectrum_oil_29/

Heartland

Couple grams of full spectrum wax, gonna be a nice night :)

Published

2 hours ago

October 24, 2020

Republished by Plato

Visit our community site for vetted suppliers at http://theCBD.place. It’s time that this subject was given more internet exposure. We are here to discuss topics related to medical marijuana and our experiences using CBD. Please do not assume that anyone here is a medical professional.

Source: https://www.reddit.com/r/CBD/comments/jhf3ch/couple_grams_of_full_spectrum_wax_gonna_be_a_nice/