All posts tagged "Bio Chemistry"

Structures of the glucocorticoid-bound adhesion receptor GPR97–Go complex

No statistical methods were used to predetermine sample size. The experiments were not randomized, and investigators were not blinded to allocation during experiments and outcome assessment....

A ‘Build and Retrieve’ methodology to simultaneously solve cryo-EM structures of membrane proteins

1. Vinothkumar, K. R. & Henderson, R. Single particle electron cryomicroscopy: trends, issues and future perspective. Q. Rev. Biophys. 49, 1–25 (2016). Article  Google Scholar  2....

Glycoproteomics

Glycoproteomics is coming of age, thanks to advances in instrumentation, experimental methodologies and computational search algorithms. Glycosylation is one of the most common post-translational modifications, and...

Molecular basis for RNA polymerase-dependent transcription complex recycling by the helicase-like motor protein HelD

Structure determination of the transcription elongation complex Bacillus subtilis is the model representative organism of the medically and industrially important Firmicutes phylum that have genomes with...

The diagnostic accuracy of isothermal nucleic acid point-of-care tests for human coronaviruses: A systematic review and meta-analysis

Search results We identified 2060 articles in total through database searching (Fig. 1). After title and abstract screening, we excluded 1941 articles that were not primary research...

A method for validating the accuracy of NMR protein structures

Outline of the method Backbone chemical shift assignments (i.e., HN, 15N, 13Cα, 13Cβ, Hα, and C′) can usually be obtained rapidly, semi-automatically, and reliably from a...

Identification and functional characterization of the first molluscan neuromedin U receptor in the slug, Deroceras reticulatum

1. Minamino, N., Kangawa, K. & Matsuo, H. Neuromedin U-8 and U-25: Novel uterus stimulating and hypertensive peptides identified in porcine spinal cord. Biochem. Biophys. Res....

The δ subunit and NTPase HelD institute a two-pronged mechanism for RNA polymerase recycling

Plasmids, DNAs, and RNAs A DNA fragment encoding B. subtilis HelD was PCR-amplified from strain MH5636 (Supplementary Table 1). The PCR product was inserted into expression vector...

Melatonin prevents doxorubicin-induced cardiotoxicity through suppression of AMPKα2-dependent mitochondrial damage

Modulation of serotonin signaling/metabolism by Akkermansia muciniphila and its extracellular vesicles through the gut-brain axis in mice

Recent research has clearly shown that serotonin, as a neurotransmitter in the gut-brain axis, can be modulated by intestinal microbiota or their metabolites in the GI...