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Propagation through alginate encapsulation of axillary buds of Cannabis sativa L. – an important medicinal plant

Cannabis sativa L. (Cannabaceae) is an important medicinal plant well known for its pharmacologic and therapeutic potency. Because of allogamous nature of this species, it is difficult to maintain its potency and efficacy if grown from the seeds. Therefore, chemical profile-based screening, selection of high yielding elite clones and their propagation using biotechnological tools is the most suitable way to maintain their genetic lines. In this regard, we report a simple and efficient method for…

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Cannabis sativa L. (Cannabaceae) is an important medicinal plant well known for its pharmacologic and therapeutic potency. Because of allogamous nature of this species, it is difficult to maintain its potency and efficacy if grown from the seeds. Therefore, chemical profile-based screening, selection of high yielding elite clones and their propagation using biotechnological tools is the most suitable way to maintain their genetic lines. In this regard, we report a simple and efficient method for the in vitro propagation of a screened and selected high yielding drug type variety of Cannabis sativa, MX-1 using synthetic seed technology. Axillary buds of Cannabis sativa isolated from aseptic multiple shoot cultures were successfully encapsulated in calcium alginate beads. The best gel complexation was achieved using 5 % sodium alginate with 50 mM CaCl2.2H2O. Regrowth and conversion after encapsulation was evaluated both under in vitro and in vivo conditions on different planting substrates. The addition of antimicrobial substance – Plant Preservative Mixture (PPM) had a positive effect on overall plantlet development. Encapsulated explants exhibited the best regrowth and conversion frequency on Murashige and Skoog medium supplemented with thidiazuron (TDZ 0.5 μM) and PPM (0.075 %) under in vitro conditions. Under in vivo conditions, 100 % conversion of encapsulated explants was obtained on 1:1 potting mix- fertilome with coco natural growth medium, moistened with full strength MS medium without TDZ, supplemented with 3 % sucrose and 0.5 % PPM. Plantlets regenerated from the encapsulated explants were hardened off and successfully transferred to the soil. These plants are selected to be used in mass cultivation for the production of biomass as a starting material for the isolation of THC as a bulk active pharmaceutical.

Keywords: Encapsulation; Nodal explants; Plant growth regulators; Plant regeneration; Synthetic seeds.

Source: https://pubmed.ncbi.nlm.nih.gov/23572915/?utm_source=no_user_agent&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1XAOX_jnjTyCUo06Xbq0lslwi760CIxcWfQx0n9x5BPH5yV1sN&fc=20200804212910&ff=20200813083454&v=2.11.5

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Industrial hemp seminar held in Chamberlain

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CHAMBERLAIN, S.D. (KELO) — About a dozen people turned out Saturday for a meeting on industrial hemp in South Dakota. 

The event was organized by producers and business people from Nebraska, where some farmers have been producing low-THC hemp. 

South Dakota is getting ready to dip its toe into the hemp field. State legislators passed a law this year legalizing hemp with no more than 0.3% THC. The U.S. Department of Agriculture approved South Dakota’s plan October 15. A state rules hearing is set for November 13. 

South Dakota Senate Democratic leader Troy Heinert of Mission started working on industrial hemp years ago. He sponsored a bill in 2016 that the House of Representatives passed but died after a Senate committee hearing. 

South Dakota lawmakers passed a bill last year but Governor Kristi Noem vetoed it. This year she agreed to sign it. 

“I’m sorry we are not as far along as we should be, because we’ve been talking about this for a long time,” Heinert said. 

What made the difference, he said, was that several tribal governments in South Dakota received USDA approval for their plans. They needed to be able to transport hemp outside their reservations. 

“Tribes were now allowed to grow hemp,” said Heinert, a member of the Rosebud Sioux Tribe. “That changed things.”  

He noted that South Dakota is only state in the nation where ingestion and possession of marijuana are felony crimes. 

Another speaker Saturday was Katie Sieverding. She is executive director for the South Dakota Industrial Hemp Association. Sieverding said the federal government wants modifications to South Dakota’s new law. She said the Noem administration is expected to bring a lengthy bill for the 2021 legislative session. 

Ken Meyer, from Winfred-based A.H. Meyer and Sons, said the company plans to use CO2 to produce CBD oil after it receives a South Dakota hemp-processor license. The company currently specializes in rendering and selling beeswax to honey producers.

Adam Brewer founded CBD Remedies in Lincoln, Nebraska. The company focuses on retail sales and also manufactures products. Brewer said packaging that includes THC data is important for consumer confidence. 

Derek Kats, who started Hemp Consultants in Lincoln, Nebraska, three years ago, said Nebraska is “very behind” in its processing for industrial hemp. “The cultivation side is key, but unless all these other pieces are put in place, the cultivation bottle-necks,” Kats said. 

Source: https://www.google.com/url?rct=j&sa=t&url=https://www.keloland.com/news/capitol-news-bureau/industrial-hemp-seminar-held-in-chamberlain/&ct=ga&cd=CAIyGjYxZmRhODRmNjY4MjhhNTU6Y29tOmVuOlVT&usg=AFQjCNGa2DXqilXAUH6NHJMLp2Owxao7OQ

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Tryplainjane 1000 mg CBD Full Spectrum Oil $29 Legit or Scam?

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I have no info on this brand. Tbh that is the price is closer to what we all should be paying for cbd. Most companies use $10 worth of cbd isolate, slap a fancy label, and maybe a dash of full spectrum and charge $100 for it. I started making my own cbd/cbg/cbn oils. Veteran grown has great prices on cbd isolate. Industrial hemp farms has the best prices on cbd cbg and cbn. 1 gram of isolate is 990 mg of cbd. Put it in warm coconut oil. Stir until dissolved and poof you have cbd oil. ✌🏻💚🌳

Edit: this is why I layer my cannaboids https://www.chiroeco.com/entourage-cbd/amp/

Source: https://www.reddit.com/r/CBD/comments/jhfjwh/tryplainjane_1000_mg_cbd_full_spectrum_oil_29/

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Couple grams of full spectrum wax, gonna be a nice night :)

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Visit our community site for vetted suppliers at http://theCBD.place. It’s time that this subject was given more internet exposure. We are here to discuss topics related to medical marijuana and our experiences using CBD. Please do not assume that anyone here is a medical professional.

Source: https://www.reddit.com/r/CBD/comments/jhf3ch/couple_grams_of_full_spectrum_wax_gonna_be_a_nice/

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