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Polyploidization for the Genetic Improvement of Cannabis sativa

Cannabis sativa L. is a diploid species, cultivated throughout the ages as a source of fiber, food, and secondary metabolites with therapeutic and recreational properties. Polyploidization is considered as a valuable tool in the genetic improvement of crop plants. Although this method has been used in hemp-type Cannabis, it has never been applied to drug-type strains. Here, we describe the development of tetraploid drug-type Cannabis lines and test whether this transformation alters yield or the…

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Cannabis sativa L. is a diploid species, cultivated throughout the ages as a source of fiber, food, and secondary metabolites with therapeutic and recreational properties. Polyploidization is considered as a valuable tool in the genetic improvement of crop plants. Although this method has been used in hemp-type Cannabis, it has never been applied to drug-type strains. Here, we describe the development of tetraploid drug-type Cannabis lines and test whether this transformation alters yield or the profile of important secondary metabolites: Δ9-tetrahydrocannabinol (THC), cannabidiol (CBD), or terpenes. The mitotic spindle inhibitor oryzalin was used to induce polyploids in a THC/CBD balanced drug-type strain of Cannabis sativa. Cultured axillary bud explants were exposed to a range of oryzalin concentrations for 24 h. Flow cytometry was used to assess the ploidy of regenerated shoots. Treatment with 20-40 μM oryzalin produced the highest number of tetraploids. Tetraploid clones were assessed for changes in morphology and chemical profile compared to diploid control plants. Tetraploid fan leaves were larger, with stomata about 30% larger and about half as dense compared to diploids. Trichome density was increased by about 40% on tetraploid sugar leaves, coupled with significant changes in the terpene profile and a 9% increase in CBD that was significant in buds. No significant increase in yield of dried bud or THC content was observed. This research lays important groundwork for the breeding and development of new Cannabis strains with diverse chemical profiles, of benefit to medical and recreational users.

Keywords: CBD; Cannabis sativa; THC; flow cytometry; polyploidy; terpenes; tetraploid; tissue culture.

Source: https://pubmed.ncbi.nlm.nih.gov/31114593/?utm_source=no_user_agent&utm_medium=rss&utm_campaign=pubmed-2&utm_content=18gXB4q-CV5o0kQDSCt3HqwNcsXbn1PxqekJlWJaIbT8zAG16G&fc=20200804213200&ff=20200813151120&v=2.11.5

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Thinking about ordering some CBD online from a reputable source, but the lab results say it has .20% THC? Can I have this shipped?

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I’m in Michigan. Really want to try this but I’m worried about shipping it to my apartment and having it get seized or getting in trouble or something.

I want to put the link to the website but I’m not sure if I can source so I’ll just say the brand is pretty reputable I just don’t know if I could get in trouble for this and what the likelihood of that would be.

Like I said it’s CBD flower but it still has larger amounts of CBD unlike most CBD in Michigan which has .3% or less (legal limit.)

Anyone think I’d be fine to order it?

Source: https://www.reddit.com/r/CBD/comments/lsgs4p/thinking_about_ordering_some_cbd_online_from_a/

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Lifter 💚 21.6% CBDa

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Visit our community site for vetted suppliers at http://theCBD.place. It’s time that this subject was given more internet exposure. We are here to discuss topics related to medical marijuana and our experiences using CBD. Please do not assume that anyone here is a medical professional.

Source: https://www.reddit.com/r/CBD/comments/lsgpsn/lifter_216_cbda/

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I believe I was scammed by Peace CBD. Has anyone heard of this brand? HELP!

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Hi, thank you for your time!

My grandparents are avid CBD users, and always order from the same brand. Around 3 months ago they received a package with a bottle of Peace CBD oil in it which they had not ordered, but they had lots of family staying at their house for Christmas and thought it must have been an order placed by somebody else. But they received another in late January, and now they realized they have been charged $100 a month, all by different billing companies across the USA, and they believe it is for these bottles of CBD. They have obviously not opened or used them, and I have had little success tracking down any information about this company online. The only posts on Reddit I was able to find under “Peace CBD” all seemed weird and scam-like, and contained the following two weird scam-like links. I was hoping someone has heard of this company or a similar scam.

(My grandparents think these scammers could have possibly gotten their information from a CBD trial they had signed up for about a year ago when they were finding a brand of CBD they liked.)

Any help or information is much appreciated!

https://sites.google.com/view/peace-cbd-oil-benefits/

https://apnews.com/press-release/newmediawire/lifestyle-business-stress-anxiety-mental-health-95498ba8a73a4c10676ba086875744b5

This last link is what comes up if you click on the attached link to “Buy Peace CBD today” on the ap article. It is not even the actual bottle of peace cbd.

https://buycannagenix.com/m/index?ts=64AAF081&s1=2503&s2=4464-8958&clickid=167390707

Thank you so much!

Source: https://www.reddit.com/r/CBD/comments/lsg4si/i_believe_i_was_scammed_by_peace_cbd_has_anyone/

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