Connect with us

Heartland

Palmitoylethanolamide and Cannabidiol Prevent Inflammation-induced Hyperpermeability of the Human Gut In Vitro and In Vivo-A Randomized, Placebo-controlled, Double-blind Controlled Trial

CONCLUSION: Cannabidiol and palmitoylethanolamide reduce permeability in the human colon. These findings have implications in disorders associated with increased gut permeability, such as inflammatory bowel disease.

Republished by Plato

Published

on

Background and aims: We aimed to examine, for the first time, the effect of cannabidiol (CBD) and palmitoylethanolamide (PEA) on the permeability of the human gastrointestinal tract in vitro, ex vivo, and in vivo.

Methods: Flux measurements of fluorescein-labeled dextrans 10 (FD10) and fluorescein-labeled dextrans 4 (FD4) dextran across Caco-2 cultures treated for 24 hours with interferon gamma (IFNγ) and tumour necrosis factor alpha (TNFα) (10 ng·mL-1) were measured, with or without the presence of CBD and PEA. Mechanisms were investigated using cannabinoid receptor 1 (CB1), cannabinoid receptor 2 (CB2), transient receptor potential vanilloid 1 (TRPV1), and proliferator activated receptors (PPAR) antagonists and protein kinase A (PKA), nitric oxide synthase, phosphoinositide 3-kinases, extracellular signal-regulated kinases (MEK/ERK), adenylyl cyclase, and protein kinase C (PKC) inhibitors. Human colonic mucosal samples collected from bowel resections were treated as previously stated. The receptors TRPV1, PPARα, PPARδ, PPARγ, CB1, CB2, G-coupled protein receptor 55 (GPR55), G-coupled protein receptor 119 (GPR119), and claudins-1, -2, -3, -4, -5, -7, and -8 mRNA were measured using multiplex. Aquaporin 3 and 4 were measured using enzyme-linked immunosorbent assay (ELISA). A randomized, double-blind, controlled-trial assessed the effect of PEA or CBD on the absorption of lactulose and mannitol in humans taking 600 mg of aspirin. Urinary concentrations of these sugars were measured using liquid chromatography mass spectrometry.

Results: In vitro, PEA, and CBD decreased the inflammation-induced flux of dextrans (P < 0.0001), sensitive to PPARα and CB1 antagonism, respectively. Both PEA and CBD were prevented by PKA, MEK/ERK, and adenylyl cyclase inhibition (P < 0.001). In human mucosa, inflammation decreased claudin-5 mRNA, which was prevented by CBD (P < 0.05). Palmitoylethanolamide and cannabidiol prevented an inflammation-induced fall in TRPV1 and increase in PPARα transcription (P < 0.0001). In vivo, aspirin caused an increase in the absorption of lactulose and mannitol, which were reduced by PEA or CBD (P < 0.001).

Conclusion: Cannabidiol and palmitoylethanolamide reduce permeability in the human colon. These findings have implications in disorders associated with increased gut permeability, such as inflammatory bowel disease.

Keywords: cannabidiol; human; intestinal; palmitoylethanolamide; permeability.

Source: https://pubmed.ncbi.nlm.nih.gov/31054246/?utm_source=no_user_agent&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1TQbL92JdzYTve0m3OsSoggMby2JyZDVBLRHiDMYZkT1hs6YNQ&fc=20200804223051&ff=20200815202906&v=2.11.5

Heartland

Republished by Plato

Published

on

Checkout PrimeXBT
Trade with the Official CFD Partners of AC Milan
Source:

Continue Reading

Heartland

Republished by Plato

Published

on

Checkout PrimeXBT
Trade with the Official CFD Partners of AC Milan
Source:

Continue Reading

Heartland

Republished by Plato

Published

on

Checkout PrimeXBT
Trade with the Official CFD Partners of AC Milan
Source:

Continue Reading
Heartland4 days ago

CBD Vape Oil Market 2021: Global Trends, Business Overview, Challenges, Opportunities …

Heartland5 days ago

Industrial Hemp in Automotive Market Estimated Forecast Analysis 2021-2030

Material4 days ago

Microstructure, characterization of interfacial phases and mechanical properties of high Nb–TiAl/Al2O3 joints brazed by novel Nb particle-reinforced Ag–Cu filler alloy

Heartland4 days ago

Drew Carey CBD – How Does It Work For Body Pain

Heartland5 days ago

National Hemp Association Awards First Recipients of Social Equity Conscious Business (SECB …

Heartland5 days ago

Heartland5 days ago

Heartland4 days ago

Natures Method CBD Gummies : How To Order & Basic Price Detail?

Heartland3 days ago

Hemp a budding industry in New Mexico

Material5 days ago

Size effect on the high strain rate micro/meso-tensile behaviors of pure titanium foil

Heartland5 days ago

Cannabis and the Autistic Spectrum

Material4 days ago

Experimental study on evolution of oxidation behavior on high-temperature continuous casting slab surface

Metal5 days ago

US rig count increases week-on-week while Canadian count declines

Heartland5 days ago

Material4 days ago

Corrosion resistance and electrical contact resistance of a thin permanganate conversion coating on dual-phase LZ91 Mg–Li alloy

Heartland3 days ago

Hello, im new to CBD and I just got this e-liquid and a pen. I looked up the vape juice after, and it doesn’t seem to exist.. Has anyone else used this brand?

Heartland5 days ago

Sleep-wake cycle disturbances and NeuN-altered expression in adult rats after cannabidiol treatments during adolescence

Heartland4 days ago

The CBD Skincare Line Nicole Kidman Swears By

Heartland4 days ago

Heartland4 days ago

Heartland4 days ago

House passes $1.9 trillion COVID relief plan

Material4 days ago

The effect of flux chemistry on element transfer in Submerged Arc Welding: application of thermochemical modelling

Metal4 days ago

ExOne names distributor in South Korea

Heartland3 days ago

Hemp a budding industry in New Mexico

Heartland2 days ago

Top 5 reasons people use CBD tinctures

Metal2 days ago

ArcelorMittal raises CRC and HDG prices in Europe

Heartland1 day ago

CBD and Menopause

Heartland5 days ago

Can cbd become ineffective?

Heartland4 days ago

https://gayle-king-cbdoil-review.medium.com/gayle-king-cbdoil-real-reviews-and

News2 days ago

Automated processing of aerospace scrap for precious elements

Trending

A Cloud Nine Capital Entity Copyright © 2020 – All Rights Reserved Proudly Made in America