Connect with us

Heartland

Miswiring the brain: Δ9-tetrahydrocannabinol disrupts cortical development by inducing an SCG10/stathmin-2 degradation pathway

Children exposed in utero to cannabis present permanent neurobehavioral and cognitive impairments. Psychoactive constituents from Cannabis spp., particularly Δ(9)-tetrahydrocannabinol (THC), bind to cannabinoid receptors in the fetal brain. However, it is unknown whether THC can trigger a cannabinoid receptor-driven molecular cascade to disrupt neuronal specification. Here, we show that repeated THC exposure disrupts endocannabinoid signaling, particularly the temporal dynamics of CB1…

Republished by Plato

Published

on

Figure 7. Δ9-tetrahydrocannabinol (THC) promotes ectopic filopodiagenesis

A,A2 Transient transfection of cortical neurons with a phospho-mimetic EGFP-SCG10S62D/S73D mutant (SCG10-DD) (Westerlund et al, 2011) led to a decrease in the primary neurite length, an effect equivalent to that of THC. SCG10-DD overexpression, which largely outcompeted endogenous SCG10 (insets), rendered THC ineffective to modify neurite elongation (> 15 neurons/condition). Western analysis demonstrated a progressive increase in SCG10-DD protein expression in PC12 cells upon transient transfection(A2). Probing by an anti-GFP antibody differentiated endogenous SCG10 from overexpressed SCG10-DD. Note that SCG10-DD overexpression significantly reduced the endogenous SCG10 content (wild-type (WT) targets around ˜20 kDa). Membranes were stained with amido-back to control for equal protein loading. B–B2 siRNA knockdown of SCG10 expression phenocopied and blunted the effects of THC on neurite outgrowth in cultured cortical neurons (> 20 neurons/condition). C SCG10 levels decreased in cortical growth cone particles exposed to THC for 10 min. SCG10 isoforms were numbered (Shin et al, 2012). Gapdh served as a loading control. D, D1 In cultured cortical neurons, THC (10 min) allowed acetylated tubulin accumulation (arrowheads) and the formation of superfluous lamellipodium-like F-actin-rich cytoskeletal projections. Bar histograms of quantitative subcellular morphometry are shown in (D2). THC facilitated the morphological differentiation of growth cones (D3) including filopodiogenesis (> 10 observations/condition; D4) without affecting the size of individual growth cones (D5). Growth cone morphology was classified as described by (Morii et al, 2006). E, E1 Besides affecting the growth cone, THC acutely increased the formation and density of F-actin+ axial protrusions, likely filopodia, from distal neurite shafts. Data information: Data were expressed as means ± s.e.m. **< 0.01, *< 0.05; n.s. = non-significant. Scale bars, 20 μm (A), 10 μm (B, D,D1,E)

Source: https://pubmed.ncbi.nlm.nih.gov/24469251/?utm_source=no_user_agent&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1z7zlNEMzQex5jriqkhCblNtckSEpT9xYlxxeymU_73UTETESY&fc=20200804213258&ff=20200811100328&v=2.11.5

Heartland

Three more Ag Things to Know

Republished by Plato

Published

on

Montana hemp update. Montana won't be implementing its USDA-approved Hemp Plan beginning Nov. 1 after all, due to the recent pilot program … Source:

Continue Reading

Heartland

COVID-19 UPDATE: CBD Oil Market 2020 Size and Growth Factors Research and Projection …

Republished by Plato

Published

on

Global CBD Oil Market report brings together a meticulous study of the present and upcoming opportunities to elucidate the future investment in the … Source:

Continue Reading

Heartland

Hemp-Based Foods Market (COVID-19 Updated) Research Report with Top Key Players- Hemp

Republished by Plato

Published

on

Hemp-Based Foods Market 2020 this report is including with the COVID19 Outbreak Impact analysis of key points influencing the growth of the market. Source:

Continue Reading
Manufacturing5 days ago

New and Noteworthy Categories on Thomasnet.com for October 2020

Heartland5 days ago

Medical Marijuana: 7 Incredible Health Benefits for Women

Heartland5 days ago

CBG Study Shows Antimicrobial Properties of Cannabis

Heartland4 days ago

German Cannabis Flower Market is Ready to Explode

Heartland2 days ago

Medical Experts Discuss Uses of CBD (via JAMA — Top Medical Journal)

Heartland5 days ago

Thanks to these CBD “cigarettes” I am a full week tobacco/nicotine free 🎉

Heartland4 days ago

Dosage for CBG/CBD tincture from LN?

Heartland5 days ago

Will I get high from a gummy with 4.5 mg cbd and .5 mg thc? I have no tolerance.

Heartland4 days ago

Question on CBD vs CBG

Heartland4 days ago

Cybin Raises C$45 Million: The Largest Go Public Capital Raise in the Canadian Psychedelic Sector

Heartland4 days ago

Indus Holdings, Inc. Welcomes Bruce Gates to Board of Directors; Announces Results of Annual Shareholder Meeting

Heartland5 days ago

National Cannabis Lobbying Falls, Again

Heartland4 days ago

Europe CBD Oil Market 2020 | Covid19 Impact Analysis | Business Outlook, Growth, Revenue …

Heartland2 days ago

Is There An Age Limit For CBD? [Is It 18 or 21?]

CBD3 days ago

CBD News Roundup: NFLPA Warns Players Not to Endorse CBD

Heartland4 days ago

Any success stories with smoking cessation?

Heartland4 days ago

How To Make CBD-Infused Face Masks At Home

Heartland4 days ago

Anyone begin CBD as a skeptic?

Heartland3 days ago

Mind Cure Announces LOI for Acquisition of Epiphany360 Digital Therapeutics Platform for Treating Mental Health

Heartland2 days ago

CBD is a life-saver for me.

Heartland1 day ago

Terping out my own cbd/cbg isolate for dabbing!! YUM!

Heartland4 days ago

BZA denies permit for hemp facility on Poplar

News4 days ago

Report Finds Legal Cannabis Could Bring Texas $500M In Annual Tax Revenue

News5 days ago

The First Medical Cannabis Dispensary in Virginia has Opened its Doors

Heartland5 days ago

The Daily Hit: October 21, 2020

Heartland4 days ago

CBD Storefront 'The Green Room' Comes To Red Bank

Heartland3 days ago

Goal of industrial hemp is to augment existing food security planning

Heartland2 days ago

Industrial hemp seminar held in Chamberlain

Material4 days ago

Microstructure and mechanical properties of 34CrNiMo6 steel repaired by laser remelting

Heartland2 days ago

Tryplainjane 1000 mg CBD Full Spectrum Oil $29 Legit or Scam?

Trending

A Cloud Nine Capital Entity Copyright © 2020 – All Rights Reserved Proudly Made in America