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Kinetics of acetylcholinesterase inhibition by hemp seed protein-derived peptides

The aim of this work was to enhance the acetylcholinesterase (AChE)-inhibitory activity of a pepsin-produced hemp seed protein hydrolysates (HPH) through reverse-phase HPLC separation followed by identification of peptide sequences present in the most active fraction. The HPH was separated into eight fractions (F1-F8) with F7 exhibiting significantly (p < 0.05) the strongest (97.5%) in vitro inhibition of electric eel AChE (eeAChE) activity in comparison to 53.8% for HPH. The HPH consisted...

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. 2019 Jul;43(7):e12897.

doi: 10.1111/jfbc.12897. Epub 2019 May 15.

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Sunday A Malomo et al. J Food Biochem. .

Abstract

The aim of this work was to enhance the acetylcholinesterase (AChE)-inhibitory activity of a pepsin-produced hemp seed protein hydrolysates (HPH) through reverse-phase HPLC separation followed by identification of peptide sequences present in the most active fraction. The HPH was separated into eight fractions (F1-F8) with F7 exhibiting significantly (p < 0.05) the strongest (97.5%) in vitro inhibition of electric eel AChE (eeAChE) activity in comparison to 53.8% for HPH. The HPH consisted mostly of low molecular weight peptides of < 11 amino acid residues and most contained at least one hydrophobic amino acid. Kinetics of enzyme inhibition revealed a mixed-type inhibition of eeAChE activity by HPH whereas F7 acted through an uncompetitive mode; in contrast inhibition of human AChE by HPH and F7 was uncompetitive. The stronger inhibitory potency of the F7 peptides fraction against both enzymes was confirmed through reduced maximal velocity, catalytic efficiency, and inhibition constant values when compared to the HPH. PRACTICAL APPLICATIONS: The use of natural products for the prevention or treatment of human diseases continues to be an area of intense research activities. Food protein-derived peptides obtained through enzymatic hydrolysis of hemp seed proteins were shown in vitro to be strong inhibitors of activities of both the eel and human forms of acetylcholinesterase (AChE). AChE is an important therapeutic target because excessive activity of this enzyme is a causative factor of neurodegenerative diseases such as dementia and Alzheimer’s. This work showed that peptides in the most active fraction are small in sizes, which may favor their absorption into blood circulation and possible permeation of the blood-brain barrier. Therefore, the hemp seed peptides are potential agents that can be used to formulate functional foods and nutraceuticals against neurodegenerative diseases.

Keywords: Alzheimer’s disease; acetylcholine; acetylcholinesterase; enzyme inhibition kinetics; hemp seed protein; protein hydrolysate; reverse-phase HPLC.

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Source: https://pubmed.ncbi.nlm.nih.gov/31353736/?utm_source=no_user_agent&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1lg0RU4SkRVNqPFA7e6OZattw-Uzcw0dBEajJBQqmhHlNZ8MdX&fc=20200804212534&ff=20200813015344&v=2.11.5

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Some Oddly Specific Predictions About Films You Should Keep an Eye Out For This Year

A collection of anticipated films that are set to be released in 2021.

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Source: https://hightimes.com/culture/movies/some-oddly-specific-predictions-films-you-should-keep-eye-out-year/

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Airfield Supply Co. And Kiva Confections Launch Breast Cancer Awareness Campaign

Two California-based companies have partnered up to launch a statewide campaign for breast cancer awareness, prevention, and research.

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Airfield Supply Co. And Kiva Confections Launch Breast Cancer Awareness Campaign | High Times






























Source: https://hightimes.com/health/airfield-supply-kiva-confections-launch-breast-cancer-awareness-campaign/

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France’s “recreational” cannabis survey • New Mexico governor prioritizes legalization • California eases banking access • & more…

The Cannabis Wire Daily newsletter is sent to C-Wire Plus subscribers every weekday morning at 7 a.m. Excerpts are published here later in the day. Don’t miss the full picture. Subscribe now. In France, parliament members enter next phase of “fact-finding mission” on cannabis. For the past year, dozens of members of parliament in France’s National Assembly have been […]

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The Cannabis Wire Daily newsletter is sent to C-Wire Plus subscribers every weekday morning at 7 a.m. Excerpts are published here later in the day. Don’t miss the full picture. Subscribe now.

In France, parliament members enter next phase of “fact-finding mission” on cannabis.

For the past year, dozens of members of parliament in France’s National Assembly have been on what they call a “fact-finding mission” about cannabis. Until recently, that mission has been focused on medical cannabis, but, now, the group has turned its attention to “recreational cannabis.”

The group released a questionnaire this week in an effort “to better understand the perception of cannabis” among the population and “their vision of the future of public policies in this area.” The questionnaire will be open until the end of February.

California regulators push toward “greater access to financial services” for the cannabis industry.

In September, as Cannabis Wirereported, several cannabis bills were signed by California Governor Gavin Newsom. Among them was Assembly Bill 1525, which, as Newsom wrote at the time, “affirmatively protects certain entities providing financial services to the legal cannabis industry … This bill has the potential to increase the provision of financial services to the legal cannabis industry, and for that reason, I support it.” 

This week, regulations to implement this bill have been published (read them here). And, as the regulations come with a proposal for “emergency action,” they are likely to quickly go into effect. 

In an email sent to stakeholders, the California Department of Food and Agriculture wrote: 

“The proposed regulations create a pathway for licensees to authorize sharing of non-public information with selected financial institutions and provide a mechanism for financial institutions to more readily conduct the federally required reviews of the cannabis business. By reducing the burden of providing financial services to cannabis businesses, more financial institutions may be willing to provide services, thereby reducing the need to keep cash on hand and improving public safety.”

New Mexico governor says cannabis is a priority.

Just as she did in January 2020, New Mexico Gov. Michelle Lujan Grisham included cannabis in her “priorities for the session” ahead of the legislature’s first day on January 19. 

As Cannabis Wirereported last January, Grisham called for cannabis legalization during her State of the State address. But even before COVID-19 hit, lawmaker opposition halted the effort.

Now, cannabis is on Grisham’s agenda again. On cannabis, her list of priorities reads:

“Establish an essential new revenue source for the state and employment source for tens of thousands of New Mexicans: Legalizing adult-use recreational cannabis through legislation that protects the state’s medical cannabis program, provides for workplace safety and roadway protections and enforcement and clear labeling of products.”

Colorado: while sales fell after mid-year, a record has been set.

Cannabis sales in the state between January and November exceeded $2 billion. Even though December data has yet to be released, the record for highest annual cannabis sales in the state was $1.75 million in 2019.

While cannabis sales steadily fell in the second half of the year, they hit an unprecedented high in the summer. 

Source: https://cannabiswire.com/2021/01/15/frances-recreational-cannabis-survey-%e2%80%a2-new-mexico-governor-prioritizes-legalization-%e2%80%a2-california-eases-banking-access-%e2%80%a2-more/

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