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Improvements in lipid suppression for 1 H NMR-based metabolomics: Applications to solution-state and HR-MAS NMR in natural and in vivo samples

Proton nuclear magnetic resonance (NMR) spectra of intact biological samples often show strong contributions from lipids, which overlap with signals of interest from small metabolites. Pioneering work by Diserens et al. demonstrated that the relative differences in diffusivity and relaxation of lipids versus small metabolites could be exploited to suppress lipid signals, in high-resolution magic angle spinning (HR-MAS) NMR spectroscopy. In solution-state NMR, suspended samples can exhibit very…

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Proton nuclear magnetic resonance (NMR) spectra of intact biological samples often show strong contributions from lipids, which overlap with signals of interest from small metabolites. Pioneering work by Diserens et al. demonstrated that the relative differences in diffusivity and relaxation of lipids versus small metabolites could be exploited to suppress lipid signals, in high-resolution magic angle spinning (HR-MAS) NMR spectroscopy. In solution-state NMR, suspended samples can exhibit very broad water signals, which are challenging to suppress. Here, improved water suppression is incorporated into the sequence, and the Carr-Purcell-Meiboom-Gill sequence (CPMG) train is replaced with a low-power adiabatic spinlock that reduces heating and spectral artefacts seen with longer CPMG filters. The result is a robust sequence that works well in both HR-MAS as well as static solution-state samples. Applications are also extended to include in vivo organisms. For solution-state NMR, samples containing significant amount of fats such as milk and hemp hearts seeds are used to demonstrate the technique. For HR-MAS, living earthworms (Eisenia fetida) and freshwater shrimp (Hyalella azteca) are used for in vivo applications. Lipid suppression techniques are essential for non-invasive NMR-based analysis of biological samples with a high-lipid content and adds to the suite of experiments advantageous for in vivo environmental metabolomics.

Keywords: 1H NMR; HR-MAS; in vivo; intact samples; lipid suppression; metabolic profiling; metabolites; solution-state NMR.

Source: https://pubmed.ncbi.nlm.nih.gov/30520113/?utm_source=no_user_agent&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1lg0RU4SkRVNqPFA7e6OZattw-Uzcw0dBEajJBQqmhHlNZ8MdX&fc=20200804212534&ff=20200814075112&v=2.11.5

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What is CBD coffee? How many drops of CBD oil in coffee can be mixed?

CBD coffee is one of the newest trends to hit the market nowadays. Most people usually start their mornings with a strong cup of coffee to energise them for the…
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CBD coffee is one of the newest trends to hit the market nowadays. Most people usually start their mornings with a strong cup of coffee to energise them for the day. Of late, health-conscious people are adding CBD to coffee to reap the benefits of a unique brew. However, for many unacquainted with this new trend, the questions remain: What is CBD Coffee? How many drops of CBD oil in coffee can be mixed?

Let’s go ahead and answer these as well as questions related to potential health benefits CBD and coffee in detail below.

What is CBD coffee?

‘CBD-infused coffee’ and ‘CBD coffee’ are some of the general terms used for coffee that has CBD added to it. CBD can be added to any type of coffee; be it a roast, a cold brew, an espresso, or a latte.

There are two ways that CBD coffee can be made. One way is that CBD oil can be added to a freshly prepared cup of coffee. Another way is that people can buy pre-infused CBD coffee grounds. Many companies nowadays are offering this innovative coffee product. In CBD infused coffee, CBD is mixed beforehand in the coffee beans during the roasting process. You simply use them to make a cup of CBD coffee.

Can you put CBD oil in coffee?

Yes, you can easily put CBD oil in coffee. CBD oil is a versatile ingredient and can be added to many beverages, coffee being one of them. Adding CBD oil to coffee is actually a very convenient way of consuming CBD and comes with many benefits. It is a discreet method to ingest CBD anytime. You can easily carry it in your bag or purse anywhere. Moreover, using CBD oil in coffee allows you to control your daily CBD dosage.

The only thing to be mindful of when adding CBD oil to coffee is not to do it in a paper cup. Paper cups may absorb the CBD oil before you get the chance to consume it.

Besides its antioxidant properties and numerous health benefits, people mostly drink coffee to benefit from caffeine. It is the active ingredient in coffee which makes you feel alert and focused. However, it might also make you feel anxious and increase your heart rate and give you the infamous coffee jitters.

CBD oil can help counter these effects. This is the main reason it is added to coffee. CBD oil is known for its calming and anxiety-reducing properties. When mixed with coffee, it provides a perfect balance between the two. You feel mellow yet alert and focused.

How many drops of CBD oil in coffee can be mixed?

The number of CBD oil drops in your coffee depends on your own personal preferences. It also depends on the strength of your CBD oil and your reasons for taking it. Generally, the recommended dose is 5-15 mg of CBD per cup. Anecdotal evidence from regular CBD coffee drinkers suggests the following preferred combinations and dosages:

  • 5-7.5 mg is a preferred dose for weekday mornings. This is because consuming CBD in small quantities has been found to have an invigorating effect. This makes it a perfect complement to a cup of a strong coffee, as it is still potent enough to overcome the unwanted coffee jitters.
  •  10-15 mg is preferred for a really soothing cup of coffee. Ideal brew for a lazy weekend.

How to make CBD coffee?

There are two common ways you can make CBD coffee. The first is by putting CBD oil in coffee after you make it. The second is by using CBD infused coffee beans. Let’s have a detailed look at both below:

1. Adding CBD oil in coffee:

To make CBD coffee this way, follow these common steps:

  • Add 6 oz of freshwater or spring water to your coffeemaker. This is better for the taste than distilled water.
  •  Decide on the amount of coffee and CBD you want. Usually, 2 teaspoons of coffee along with 5 mg of CBD are enough for a 6-ounce cup of coffee. However, feel free to adjust this to your liking.
  • Add the coffee to the coffee maker and brew your coffee. You can also add milk and syrups depending on your taste.
  • Now that your coffee is prepared, add a few drops of CBD oil to it according to your preferred dosage. Mix the coffee with a spoon or a frother to combine all the ingredients well.

2. Using CBD-infused coffee beans:

To make CBD coffee this way, follow these common steps:

  • Add around 10-15 g of ground CBD coffee to your cafetiere or French press.
  •  Measure and add boiling water (around 6-ounces)
  •  Stir and wait around 2-3 minutes
  • In the last step, gently push the plunger/ strainer down and pour yourself a cup of hot CBD coffee.

CBD – Infused Coffee

Both Coffee and CBD have their own set of benefits individually. When combined, they can act as a powerhouse of health support, according to anecdotal evidence from people who consume CBD coffee regularly.

CBD is known for it’s anti-inflammatory and anxiety-reducing properties. Coffee is rich in antioxidants too. A cup of CBD coffee in the morning can therefore help with managing chronic pain and assist in muscle recovery from a strenuous workout.

The infusion of CBD in coffee makes a perfect between CBD and caffeine. You not only reap the benefits of Vitamins B1, B2 and Vitamin D, omega-3 and omega 6- fatty acids, you also feel much calmer and focused. CBD oil successfully counteracts the side effects of caffeine such as jitteriness, muscle cramps and increased anxiety.

Moreover, CBD works well in tandem with the caffeine in coffee. When combined, they both increase the duration of the effects of one another. This is beneficial for CBD coffee consumers as they feel heightened levels of calm productivity and alertness for longer periods of time.

Another point to note is that since CBD has no addictive or psychoactive qualities, CBD coffee is non-addictive and will not get you ‘high’.

However, like all good things, CBD coffee should be consumed in moderation. To experience the benefits of CBD coffee discussed above, no more than 2 cups of CBD infused coffee should be taken in a day.

Key Takeaways

CBD coffee in the UK is gaining popularity fast. Many cafés are offering a variety of CBD infused coffees, ranging from cold brews to espressos and lattes. CBD oil and coffee work well together. Anecdotal evidence suggests that when combined, they both offer a host of health benefits such as reducing anxiety and enhancing mental alertness.

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Source: https://www.theextract.co.uk/lifestyle/what-is-cbd-coffee/

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Women promote cannabis use and marijuana cultivation

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The cannabis world is also purple: they are mothers, activists, professionals from different areas, entrepreneurs, cultivators, consumers. They are cannabis women.

The feminist tide that runs through Latin America, as well as the debates over the legalization of cannabis in the region, has given them visibility. In Mexico, their voices are increasingly present in the streets, on social networks, through collectives and associations that promote the medicinal or recreational use of the plant, spaces from which they also seek to break with stigmas and demand legalization with a gender perspective.

Karen growcia is one of them. Three years ago, tired of being stigmatized and taking risks for clandestinely obtaining marijuana, she decided to adapt her backyard and a corner indoors to grow her own plants. « Because of the need to stop risking my life by going to look for cannabis in places where they are not recommended for a woman and to stop consuming things that we do not know what they have, that is why my desire to start growing », he says.

A member of the Puerto Rican collective YunqueCrew, mother of an eight-year-old girl and a consumer for 10 years, she has found in cannabis a way to cope with exhausting maternal care and menstrual pain: “as a mother, one suffers a lot of stress; you are undertaking and you are very moved, this serves for stress. In menstrual cycles, instead of taking a pill, I smoke a joint or use CBD, the medicinal part, to calm my nerves and pains ”.

Jesica Maravilla « marnúñ », who participates taking care of the garden in the 420 plant installed outside the Senate of the Republic for a year, also considers that it is a plant that nourishes the female body.

Others have been enjoying its medicinal benefits for years, even if that means going against the medical structure. It is the case of Erandeni « Gi », who has been using marijuana for eight years to control epilepsy. This girl who collaborates in the social networks of the 420 plant says that it was her doctor who unofficially suggested drinking it in tea to alleviate insomnia and epileptic seizures, then she discovered the oil that changed her life.

« Little by little I was able to stop the medication without having problems, I have a good life and I am not taking other alternative medications. I don’t know if it works for everyone, but it does for me, and it is important that others understand the benefits, » he says.

Spreading the healing properties of cannabis and debunking the myths that demonize it is precisely the mission of The Sisters of the Valley, a movement of cannabis “nuns” born in California, which has partners in Mexico and other countries. For four years, the sisters Camilla and Luna They took the habit that characterizes the group and has dedicated themselves to promoting and marketing medicinal products in the country.

Wearing the habit is symbolic, they are not religious, but their life is governed by respect for nature, and the elaboration of their products follows a particular ritual: “Our plants and products are made under lunar cycles, it is part of our rules and commitments; It is medicine and we care that it has the best of us; we do ceremonies between lunar cycles and the production of our medicine ”, he explains Camilla Valley, activist and founder of the group in Mexico.

Its medicinal properties are mainly anti-inflammatory, which is why it acts against joint pain, migraines and menstrual cramps, explains Luna, who also studies biomedicine.

From other international associations such as Mother Cultivate ‘Mothers from different Latin American countries whose children suffer from epilepsy or other pathologies have been fighting for years so that access to cannabis oil, seeds of the plant and their self-cultivation are decriminalized. Thus they have also managed to weave a network of collective care.

In Texcoco, Mariana lopez he has woven his own self-care networks with marijuana. Buds grow in her home garden that she uses for home remedies. He started preparing them to relieve their migraines and joint pains, now he makes creams and oils that he then distributes to his neighbors and mothers with children with ADHD (attention deficit hyperactivity disorder).

There is no doubt that in the country, even though legalization is still pending, there is a booming cannabis industry, and women are putting their own stamp on it. Medicinal, therapeutic, feminine hygiene products, to promote sexual pleasure, cosmetics. “What are they not doing! There are girls making lubricants, there are different themes, and they are all betting on the first step, which is not being afraid to recognize themselves and to be the front line of cannabis women who are fighting for all, « he says Cati Villarreal, cultural manager and member of the women’s circles that organize every Wednesday at the Reforma sit-in.

In that weekly space, Cati and other girls have brought together 11 cannabis women’s organizations for the first time to share experiences and create solidarity networks. From there they also seek to sensitize the cannabis movement to the feminist struggle and demand legalization with a gender perspective, since proposals such as the bill that is currently being reviewed in Congress leave those who self-cultivate for medicinal or recreational use at a disadvantage.

They are marking more stigmatization, says Karen: “I have a daughter and the new law is telling me that I cannot grow or consume if I have a minor at home, although I have a space with a patio so I don’t do it where he is, and He also tells me that I cannot smoke on the street, instead of helping, he is stigmatizing and criminalizing us more ”.

Anyone should grow what they consume without being labeled a criminal, he maintains. In her opinion, the law under discussion will benefit only large companies: “If I, as a mother cultivator, want to start a business, I will find it very difficult because I will not have the money to buy a permit and be able to get a product, a mark ».

Both the bill and the recently published regulations governing the medicinal use of marijuana do not take into account entrepreneurial women or indigenous peasant women or producers.

« How is it possible that the regulation only contemplates five or six plants and if they find a seventh I will already be a drug trafficker? », Asks Cati, who also points out the void in which this law would leave peasant women or indigenous women who cultivate and benefit from the plant from their traditional knowledge.

“The female deputies are not talking about cannabis women and what they use them for, some are mothers taking care of their children. They are going to have to request more than five licenses to cure their children.  » She assures that they are leaving vulnerable groups of women at a disadvantage, such as the elderly, peasant women or single mothers.

The Sisters of the Valley México see advances in regulation, but also criticize that it only benefits a few. In addition, they question that the regulation of medicinal use published on January 1 indicates that any promotion of cannabis medicine is directed only to health professionals, not to the general population.

“It makes me serious that you can’t promote it because that limits very valuable information that we all need to know, and we as activists have the job to talk about the benefits of the plant. It’s not fair to me, ”says Camilla Valley.

FS

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Source: https://www.google.com/url?rct=j&sa=t&url=https://www.explica.co/women-promote-cannabis-use-and-marijuana-cultivation/&ct=ga&cd=CAIyGmM2M2RhZjlmZTVmZDZjMmU6Y29tOmVuOlVT&usg=AFQjCNGzGdF81ZLun1qe4j3d4I8hklL-Bg

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Women promote cannabis use and marijuana cultivation

Republished by Plato

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… to cannabis oil, seeds of the plant and their self-cultivation are decriminalized. Thus they have also managed to weave a network of collective care. Checkout PrimeXBT
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