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Hemp seed polysaccharides protect intestinal epithelial cells from hydrogen peroxide-induced oxidative stress

The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H(2)O(2)-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection. Analysis of monosaccharide composition and structure of two fractions HSP(0) and HSP(0.2) from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR). The results showed that both…

Published

2 years ago

May 21, 2019

Republished by Plato

. 2019 Aug 15;135:203-211.

doi: 10.1016/j.ijbiomac.2019.05.082. Epub 2019 May 17.

Affiliations

¹ Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China. Electronic address: zswenmr@163.com.
² Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.
³ Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China; School of Marine Science and Technology, Zhejiang Ocean University, Zhejiang 316022, China.
⁴ Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China; Zhejiang Marine Development Research Institute, Zhoushan, Zhejiang 316021, China. Electronic address: xxw11086@126.com.
⁵ Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China; Zhejiang Marine Development Research Institute, Zhoushan, Zhejiang 316021, China.

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Zheng-Shun Wen et al. Int J Biol Macromol. 2019.

. 2019 Aug 15;135:203-211.

doi: 10.1016/j.ijbiomac.2019.05.082. Epub 2019 May 17.

Affiliations

¹ Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China. Electronic address: zswenmr@163.com.
² Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.
³ Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China; School of Marine Science and Technology, Zhejiang Ocean University, Zhejiang 316022, China.
⁴ Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China; Zhejiang Marine Development Research Institute, Zhoushan, Zhejiang 316021, China. Electronic address: xxw11086@126.com.
⁵ Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China; Zhejiang Marine Development Research Institute, Zhoushan, Zhejiang 316021, China.

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Abstract

The purpose of this study was to investigate structure of Hemp seed polysaccharide (HSP) and the protective effect of HSP from H₂O₂-induced oxidative damage in IPEC-1 cells and the possible mechanism of this protection. Analysis of monosaccharide composition and structure of two fractions HSP₀ and HSP_0.2 from polysaccharide of Hemp seed (HSPc) were analyzed by high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FT-IR). The results showed that both HSP₀ and HSP_0.2 contain sulfate groups, which are sulfated polysaccharides. In IPEC-1 cells model, the release of LDH and MDA was significantly decreased, and the activities of SOD, GSH-Px and CAT were significantly increased in HSP₀ and HSP_0.2-treated group. HSP_0.2 dramatically increased the gene expression of antioxidant enzymes and phase II detoxification enzymes measured by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (qRT-PCR). In addition, HSP_0.2 up-regulated the expression level of intracellular transcription factor Nuclear factor erythroid-2-related factor 2 (Nrf2) and inhibited the level of Kelch-like ECH-associated protein 1 (Keap1) with Western blot analysis. Collectively, the present study suggested that HSP_0.2 has the protective effect of IPEC-1 cells against H₂O₂-induecd oxidative stress. This protection mechanism may be related to activation of the Keap1/Nrf2 signaling pathway.

Keywords: Hemp seed polysaccharide; Keap1/Nrf2 signaling pathway; Oxidative stress.

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