Sugars streams generated by organosolv pretreatment of hemp hurds, cellulose (C6) and hemicellulose (C5) fractions, were fermented to lactic acid (LA) by Bacillus coagulans strains XZL4 and DSM1. Pretreatment conditions and enzymatic hydrolysis were optimized and B. coagulans aptness to use lignocellulosic-derived sugars as a carbon source was evaluated. Methanolic organosolv pretreatment with 2.5% (w/w) H2SO4 gave the best results in terms of glucan recovery (98%), enzymatic hydrolysis of pretreated biomass (70%) and hemicellulosic sugars recovery (61%). C6 and C5 sugars fermentation by strain XZL4 gave, high LA yields (0.90 and 0.84 g/g), high titers (141 and 109 g/L), and high enantiomeric excess (>99%). Overall, 42 g of l-LA were obtained from 100 g of raw hemp hurds. These results can be considered promising for lignocellulosic feedstock valorization toward the production of polymer-grade LA.
Keywords: Biomass conversion; Biorefineries; Hemp hurds; Lactic acid; Organosolv.
Copyright © 2015 Elsevier Ltd. All rights reserved.
Cited by 1 article
Single and Mixed Feedstocks Biorefining: Comparison of Primary Metabolites Recovery and Lignin Recombination During an Alkaline Process.
Front Chem. 2020 Jun 5;8:479. doi: 10.3389/fchem.2020.00479. eCollection 2020. Front Chem. 2020. PMID: 32582644 Free PMC article.
How long does it take for CBD to work?
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Plus Products Restructures Convertible Debt
Plus Products Inc. Announces Debentureholder Approval of Debenture Amendments SAN MATEO, Calif., Feb. 25, 2021 (GLOBE NEWSWIRE) — Plus Products Inc. (CSE: PLUS) (OTCQX: PLPRF) (the “Company” or “PLUS”), a cannabis and hemp branded products company in the U.S., is pleased to announce today that, further to its press release dated February 3, 2021, holders […]
Plus Products Inc. Announces Debentureholder Approval of Debenture Amendments
SAN MATEO, Calif., Feb. 25, 2021 (GLOBE NEWSWIRE) — Plus Products Inc. (CSE: PLUS) (OTCQX: PLPRF) (the “Company” or “PLUS”), a cannabis and hemp branded products company in the U.S., is pleased to announce today that, further to its press release dated February 3, 2021, holders (the “Debentureholders”) of the Company’s 8.00% unsecured convertible debentures due February 28, 2021 (the “Debentures”) have approved by way of signed instruments in writing in the form of the Form of Proxy, certain proposed amendments to the terms of the Debentures (the “Debenture Amendments”) and proposed resolutions for consideration (the “Resolution Amendments” and together with the Debenture Amendments, the “Amendments”). The Amendments have therefore been validly approved.
As a result of such approval, the extraordinary meeting of Debentureholders that was to be held today, February 25, 2021, in respect of the Amendments has been cancelled.
A Management Information Circular dated January 26, 2021 (the “Circular”) containing a detailed description of the Amendments has been mailed to the Company’s Debentureholders of record.
As a result of such approval, the Debenture Amendments will:
a.) extend the maturity date of the Debentures from February 28, 2021 to February 28, 2024;
b.) increase the coupon rate of the Debentures from 8.00% to 12.00% per annum effective February 28, 2021;
c.) grant Odyssey Trust Company, on its behalf and on behalf of the Debentureholders, a first charge security interest in all of the Company’s present and after acquired properties;
d.) grant a conversion right to the holder of a Debenture pursuant to which Debentureholders may require the Company to convert their outstanding Debentures pro rata up to a maximum amount of CAD$6,250,000 (representing 25%) of the principal amount of the Debentures to be so converted at a conversion price of CAD$0.95, exercisable up to March 31, 2021, and delete the Debentureholder’s right to convert any part of the principal amount of a Debenture into common shares at a price of CAD$6.50 in the capital of the Company; and
e.) amend the redemption price to an amount equal to 103% of the principal amount, including accrued and unpaid interest thereon up to (and including) the redemption date up to February 28, 2023 and thereafter until the maturity date to an amount equal to 101.50% of the principal amount, including accrued and unpaid interest thereon up to (and including) the redemption date.
In addition, the Resolution Amendments approved include the:
a) payment of a consent fee equal to CAD$499 for every CAD$1,000 principal amount of Debentures held by such Debentureholders as at the record date and to be paid in the form of common share purchase warrants (the “Consent Fee”) to those Debentureholders having consented to the Debenture Amendments on or before February 22, 2021. In consideration of the Consent Fee paid to such consenting Debentureholders, the Company shall issue to the Debentureholders one (1) common share purchase warrant (each, a “Warrant”) for every CAD$1.10 Consent Fee consideration to be received by Debentures held by such Debentureholders as at the record date.
For greater certainty, the total aggregate consideration of such Consent Fee shall be 454 Warrants for every CAD$1,000 principal amount of Debentures issued to those Debentureholders having consented to the Debenture Amendments on or before February 22, 2021.
Each whole Warrant shall entitle the holder thereof to purchase one (1) Subordinate Voting Share at a price of CAD$1.10 per share at any time on or before February 28, 2024;
b) delisting of the Debentures from the Canadian Securities Exchange (the “CSE”);
c) listing of the amended Debentures on the CSE.
The Company will apply to delist the Debentures from the CSE. The Company will subsequently apply to list the amended Debentures on the CSE.
Conversion Rights Procedure
In connection with the Amendments, holders of the amended Debentures are afforded the right to convert their outstanding amended Debentures pro rata up to a maximum amount of CAD$6,250,000 (representing 25%) of the principal amount of the amended Debentures, at a conversion price of CAD$0.95 per Subordinate Voting Share (the “Conversion Price”).
Commencing March 1, 2021, a holder of the amended Debentures shall have the right, at their option, to require PLUS to convert the holder’s amended Debentures into Subordinate Voting Shares at a price equal to the Conversion Price and to be completed upon the expiry date of March 31, 2021 (the “Conversion Date”). Each holder of amended Debentures who elects to have their amended Debentures converted is required to notify Odyssey Trust Company, as trustee (the “Trustee”), prior to 4:00 p.m. (Pacific time) on March 29, 2021, being two Business Days prior to the Conversion Date.
The notice required to be provided to the Trustee shall be in the form attached to the supplemental indenture to be entered into between PLUS and the Trustee (the “Supplemental Indenture”) and filed on the Company’s SEDAR profile as Schedule “B” (the “Conversion Notice”).
The Conversion Notice, when received by the Trustee from a holder, shall be irrevocable.
PLUS shall not be required to convert an aggregate principal amount on the Conversion Date of more than CAD$6,250,000, in respect of the aggregate principal amount of amended Debentures (the “Maximum Conversion Amount”) tendered pursuant to the conversion right. In the event that more than the Maximum Conversion Amount of Debentures are so tendered for conversion, PLUS shall convert the Debentures tendered pro rata up to the Maximum Conversion Amount.
The Conversion Right will not affect the rights of those Debentureholders who have not accepted it. Debentureholders who have not accepted the Conversion Right will not be entitled to the benefit and will not receive the Subordinate Voting Shares issuable upon conversion of the amended Debentures, and will retain their full rights under the amended Debentures.
The securities issued pursuant to the conversion noted in this press release are subject to a statutory hold period of four (4) months from the date of issuance. Consequently, unless otherwise permitted by applicable securities legislation, the holder of such securities must not trade the securities before such date.
Copies of the Management Information Circular and Supplemental Indenture can be found in the Company’s disclosure documents on the SEDAR website at www.sedar.com.
PLUS is a cannabis branded products company focused on using nature to bring balance to consumers’ lives. PLUS’s mission is to make cannabis safe and approachable – that begins with high-quality products that deliver consistent consumer experiences. PLUS is headquartered in San Mateo, CA.
For fact-based information on Plus Products, view the company’s sponsored Investor Dashboard.
Get ahead of the crowd by signing up for 420 Investor, the largest & most comprehensive premium subscription service for cannabis traders and investors since 2013.
What am I feeling on CBD?
Long story short, I am trying CBD again after a traumatic experience with THC cannabis years ago.
My issue, when I try CBD flower is if anything feels familiar about the effects, I start to freak out that I am going to have a bad trip again.
With that said, I need your help navigating through my though process when I smoke CBD and ways that I can ease my mind about what I am experiencing and relax when I feel those effects knowing they are safe and no indication I am going to trip like I do on thc.
Body feels like i just put on a sweater. That is the only way I can describe it. Not like I feel warm or anything. It’s more like I can feel the muscles in my shoulders, chest and arms. When I smoke cbd this is one of the first effects I get and it trips me out because I worry that what i am feeling is my muscles tightening due to an anxiety response from the trace amounts of thc. So I never know if I am feeling a good effect of the cbd muscle relaxing properties or the anxiety induced muscle tightening from the thc.
Feeling spacey. I notice if I smoke enough CBD flower, I will get a little absent minded. I don’t have thoughts racing through my head but I almost get that fogginess and forgetfulness that I used to get with thc flower. It’s way more subtle with CBD but it’s enough that I start to psych myself out that I am about to trip the same way I do when I smoked thc flower.
Altered first person view. I couldn’t really give it a better name. It sounds trippier than what I really mean, I promise. When I would smoke thc flower I noticed that the way things looked would change. Not in any sort of extreme way, maybe colors where a bit more vibrant or things seemed to be a bit cloudy. They way I described it back then was like wearing an astronaught helmet. It just always seem like you were looking at the world from behind a glass screen. Not literally but that’s the best I can explain it. You never really felt present. You felt more inside of yourself, looking out from the inside than actually being part of the moment. I used to love that feeling. Well with CBD I certainly don’t get anywhere near that feeling but I do notice a small shift in my first person view. So it makes me freakout thinking I might be tripping on the THC and will have a bad trip like those time.
Last one is more my fault but i’ll list it here anyway. I will question my thoughts when I’ve smoked CBD. Any thought that is mildly complex immedietly gets question as “was that a high thought?” “if it was then we might be too high and am going to start spiraling”. I do it for damn near any thought. It also happens if I read something that is just a bit worry some, i’ll start to trip out thinking that I’m possibly experiencing that thc effect where it makes things feel more impactful and serious than they really are.
All of these things contribute to me experiencing anxiety when I smoke CBD. If I am being objective, what I experience on CBD, though familiar, isn’t perfectly controllable if I can just learn that not every change in my mental states or experience on CBD means I am high off of thc. I just have a fear that smoking is gonna flip a switch in my brain and make me schizophrenic like my uncle was (not to overshare). I fear that maybe there is enough thc in these things to cause that trigger to happen and what I am experiencing on CBD is an indication that i am tripping on thc while will lead me to going crazy.
I hope you all have some advice for me on how to navigate these thoughts and realize that I am okay and it’s nothing like thc.
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