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Genipin-Aided Protein Cross-linking to Modify Structural and Rheological Properties of Emulsion-Filled Hempseed Protein Hydrogels

Genipin, a natural electrophilic cross-linker, was applied (5, 10, 20, and 30 mM) to modify hempseed protein isolate (HPI). Genipin treatments resulted in general losses of total sulfhydryls (up to 2.9 nmol/mg) and free amines (up to 77.3 nmol/mg). Surface hydrophobicity decreased by nearly 90% with 30 mM genipin, corresponding to similar tryptophan fluorescence quenching. The genipin treatment converted HPI into highly cross-linked polymers. Hydrogels formed with such polymers when also…

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Genipin, a natural electrophilic cross-linker, was applied (5, 10, 20, and 30 mM) to modify hempseed protein isolate (HPI). Genipin treatments resulted in general losses of total sulfhydryls (up to 2.9 nmol/mg) and free amines (up to 77.3 nmol/mg). Surface hydrophobicity decreased by nearly 90% with 30 mM genipin, corresponding to similar tryptophan fluorescence quenching. The genipin treatment converted HPI into highly cross-linked polymers. Hydrogels formed with such polymers when also incorporated with hemp oil emulsions exhibited substantially enhanced gelling ability: up to 3.3- and 2.6-fold increases, respectively, in gel strength and gel elasticity over genipin-untreated protein. The genipin-modified composite gels also exhibited superior water-holding capacity. Microstructural analysis revealed a compact gel network filled with protein-coated oil globules that interacted intimately with the protein matrix when treated with genipin. Such gels remained readily digestible. Hence, genipin-treated hemp protein hydrogels show promise as functional food components.

Keywords: composite gel; cross-linking; genipin; hemp protein.

Source: https://pubmed.ncbi.nlm.nih.gov/31682429/?utm_source=no_user_agent&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1lg0RU4SkRVNqPFA7e6OZattw-Uzcw0dBEajJBQqmhHlNZ8MdX&fc=20200804212534&ff=20200812055240&v=2.11.5

Heartland

Scraped this blob of sticky stuff off the blades of our trimmer machine and put it in a pocket of my backpack about a month ago then forgot about it. Just remembered it.

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Visit our community site for vetted suppliers at http://theCBD.place. It’s time that this subject was given more internet exposure. We are here to discuss topics related to medical marijuana and our experiences using CBD. Please do not assume that anyone here is a medical professional.

Source: https://www.reddit.com/r/CBD/comments/jkrdav/scraped_this_blob_of_sticky_stuff_off_the_blades/

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Heartland

Why do smoke shops only sell shitty cbd carts?

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Primal nation wide has good ones. Anything in shops is either expensive as fuck or garbage so you buy the expensive stuff in the future… primal has Cbd you can make carts with super cheap right now but his carts are also like buy one get one 50 percent off I think. I think 30 for 2 gram carts

Source: https://www.reddit.com/r/CBD/comments/jkoody/why_do_smoke_shops_only_sell_shitty_cbd_carts/

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Heartland

We will compile a list for black friday

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We will compile a list for black friday of all the deals going on.

We already have some compiled, such as 40% off big sky bontanicals or theCBDistillery.

Please don’t make posts about black friday deals – but instead we will release our post a few days before and you can post the discounts there.

Remember – do not share links. It violates the rules.

Source: https://www.reddit.com/r/CBD/comments/jknp8z/we_will_compile_a_list_for_black_friday/

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