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Endoplasmic reticulum retention signaling and transmembrane channel proteins predicted for oilseed ω3 fatty acid desaturase 3 (FAD3) genes

Oilseed crop oils contain a variety of unsaturated fatty acids that are synthesized and regulated by fatty acid desaturases (FADs). In this study, 14 FAD3 (ω3 desaturase) protein sequences from oilseeds are analyzed and presented through the application of several computational tools. The results indicated a close relationship between Brassica napus and Camelina sativa, as well as between Salvia hispanica and Perilla frutescens FAD3s, due to a high similarity in codon preferences in codon usage…

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Oilseed crop oils contain a variety of unsaturated fatty acids that are synthesized and regulated by fatty acid desaturases (FADs). In this study, 14 FAD3 (ω3 desaturase) protein sequences from oilseeds are analyzed and presented through the application of several computational tools. The results indicated a close relationship between Brassica napus and Camelina sativa, as well as between Salvia hispanica and Perilla frutescens FAD3s, due to a high similarity in codon preferences in codon usage clusters and the phylogenetic tree. The cis-acting element results reveal that the seed-specific promoter region of BnFAD3 contains the critical conserved boxes such as HSE and ABRE, which are involved in responsiveness to heat stress and abscisic acid. The presence of the aforementioned conserved boxes may increase cold acclimation as well as tolerance to drought and high salinity. Omega(ω)3 desaturases contain a Skn-1 motif which is a cis-acting regulatory element required involved in endosperm development. In oilseed FAD3s, leucine is the most repeated amino acid in FAD3 proteins. The study conveyed that B. napus, Camelina sativa, Linum usitatissimum, Vernicia fordii, Gossypium hirsutum, S. hispanica, Cannabis sativa, and P. frutescens have retention signal KXKXX/XKXX at their c-terminus sites, which is one of the most important characteristics of FADs. Additionally, it was found that BnFAD3 is a transmembrane protein that can convert ω6 to ω3 fatty acids and may simultaneously act as a potassium ion channel in the ER.

Keywords: Codon usage; Conserved histidine; Delta 15 desaturase; Ion channel; Retention signal.

Source: https://pubmed.ncbi.nlm.nih.gov/31781992/?utm_source=no_user_agent&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1lg0RU4SkRVNqPFA7e6OZattw-Uzcw0dBEajJBQqmhHlNZ8MdX&fc=20200804212534&ff=20200812055240&v=2.11.5

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Some Oddly Specific Predictions About Films You Should Keep an Eye Out For This Year

A collection of anticipated films that are set to be released in 2021.

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Some Oddly Specific Predictions About Films You Should Keep an Eye Out For This Year | High Times




























Source: https://hightimes.com/culture/movies/some-oddly-specific-predictions-films-you-should-keep-eye-out-year/

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Airfield Supply Co. And Kiva Confections Launch Breast Cancer Awareness Campaign

Two California-based companies have partnered up to launch a statewide campaign for breast cancer awareness, prevention, and research.

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Airfield Supply Co. And Kiva Confections Launch Breast Cancer Awareness Campaign | High Times






























Source: https://hightimes.com/health/airfield-supply-kiva-confections-launch-breast-cancer-awareness-campaign/

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France’s “recreational” cannabis survey • New Mexico governor prioritizes legalization • California eases banking access • & more…

The Cannabis Wire Daily newsletter is sent to C-Wire Plus subscribers every weekday morning at 7 a.m. Excerpts are published here later in the day. Don’t miss the full picture. Subscribe now. In France, parliament members enter next phase of “fact-finding mission” on cannabis. For the past year, dozens of members of parliament in France’s National Assembly have been […]

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The Cannabis Wire Daily newsletter is sent to C-Wire Plus subscribers every weekday morning at 7 a.m. Excerpts are published here later in the day. Don’t miss the full picture. Subscribe now.

In France, parliament members enter next phase of “fact-finding mission” on cannabis.

For the past year, dozens of members of parliament in France’s National Assembly have been on what they call a “fact-finding mission” about cannabis. Until recently, that mission has been focused on medical cannabis, but, now, the group has turned its attention to “recreational cannabis.”

The group released a questionnaire this week in an effort “to better understand the perception of cannabis” among the population and “their vision of the future of public policies in this area.” The questionnaire will be open until the end of February.

California regulators push toward “greater access to financial services” for the cannabis industry.

In September, as Cannabis Wirereported, several cannabis bills were signed by California Governor Gavin Newsom. Among them was Assembly Bill 1525, which, as Newsom wrote at the time, “affirmatively protects certain entities providing financial services to the legal cannabis industry … This bill has the potential to increase the provision of financial services to the legal cannabis industry, and for that reason, I support it.” 

This week, regulations to implement this bill have been published (read them here). And, as the regulations come with a proposal for “emergency action,” they are likely to quickly go into effect. 

In an email sent to stakeholders, the California Department of Food and Agriculture wrote: 

“The proposed regulations create a pathway for licensees to authorize sharing of non-public information with selected financial institutions and provide a mechanism for financial institutions to more readily conduct the federally required reviews of the cannabis business. By reducing the burden of providing financial services to cannabis businesses, more financial institutions may be willing to provide services, thereby reducing the need to keep cash on hand and improving public safety.”

New Mexico governor says cannabis is a priority.

Just as she did in January 2020, New Mexico Gov. Michelle Lujan Grisham included cannabis in her “priorities for the session” ahead of the legislature’s first day on January 19. 

As Cannabis Wirereported last January, Grisham called for cannabis legalization during her State of the State address. But even before COVID-19 hit, lawmaker opposition halted the effort.

Now, cannabis is on Grisham’s agenda again. On cannabis, her list of priorities reads:

“Establish an essential new revenue source for the state and employment source for tens of thousands of New Mexicans: Legalizing adult-use recreational cannabis through legislation that protects the state’s medical cannabis program, provides for workplace safety and roadway protections and enforcement and clear labeling of products.”

Colorado: while sales fell after mid-year, a record has been set.

Cannabis sales in the state between January and November exceeded $2 billion. Even though December data has yet to be released, the record for highest annual cannabis sales in the state was $1.75 million in 2019.

While cannabis sales steadily fell in the second half of the year, they hit an unprecedented high in the summer. 

Source: https://cannabiswire.com/2021/01/15/frances-recreational-cannabis-survey-%e2%80%a2-new-mexico-governor-prioritizes-legalization-%e2%80%a2-california-eases-banking-access-%e2%80%a2-more/

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