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Distribution of Lignin, Hemicellulose, and Arabinogalactan Protein in Hemp Phloem Fibers

The distribution of lignin, 8-5′ and 8-8′ linked lignin substructure, and noncellulosic polysaccharides in hemp (Cannabis sativa L.) phloem fibers were explored based on histochemical and immunological methods. Ultraviolet absorption and potassium permanganate staining were observed mainly in the compound middle lamella (CML) and S1 layers, and rarely in the G-layer of phloem fibers, suggesting that lignin concentration is high at the CML and S1 layers, and very low at the G-layer of hemp…

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The distribution of lignin, 8-5′ and 8-8′ linked lignin substructure, and noncellulosic polysaccharides in hemp (Cannabis sativa L.) phloem fibers were explored based on histochemical and immunological methods. Ultraviolet absorption and potassium permanganate staining were observed mainly in the compound middle lamella (CML) and S1 layers, and rarely in the G-layer of phloem fibers, suggesting that lignin concentration is high at the CML and S1 layers, and very low at the G-layer of hemp fibers. Acriflavine staining, uniform KM1 labeling (8-5′ linked lignin substructure), and no KM2 labeling (8-8′ linked structure) were observed in the G-layer, suggesting that there is a small amount of lignin-like compound with 8-5′ linked structure in the G-layer. In addition, some fiber cells showed a multilayered structure. Uniform arabinogalactan protein (AGP) labeling was observed on the S1 layers and G-layers using JIM14, but little appeared in the CML of hemp fibers, indicating that these layers of the phloem fibers contain AGP. Immunogold labeling of xylan (LM11) and glucomannan (LM21) showed that xylan and glucomannan were mainly present in the S1 layers and the G-layers, respectively. In some phloem fibers, LM21 immunofluorescence labeling showed multilayered structure, suggesting the heterogeneous distribution of glucomannan.

Keywords: Cannabis sativa L.; 8-5′ linkage; 8-8′ linkage; lignin; monoclonal antibody.

Source: https://pubmed.ncbi.nlm.nih.gov/30175708/?utm_source=no_user_agent&utm_medium=rss&utm_campaign=pubmed-2&utm_content=18gXB4q-CV5o0kQDSCt3HqwNcsXbn1PxqekJlWJaIbT8zAG16G&fc=20200804213200&ff=20200814143111&v=2.11.5

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Rule #1 no link to vendors, first post is link to vendors

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Visit our community site for vetted suppliers at http://theCBD.place. It’s time that this subject was given more internet exposure. We are here to discuss topics related to medical marijuana and our experiences using CBD. Please do not assume that anyone here is a medical professional.

Source: https://www.reddit.com/r/CBD/comments/jiwv35/rule_1_no_link_to_vendors_first_post_is_link_to/

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Oil vs gummies

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I sometimes use CBD to help me sleep, but something is confusing me. I have tried oils like Feals, which all seem to have about 25mg – 40mg of CBD. But then I also have gummies (like Plus) that tend to have 5mg – 10mg (plus .5mg – 1mg THC) and work just as well for me.

Is that small amount of THC enough to make a big enough difference to require so much less CBD? Or is it related to the gummy vs oil?

Source: https://www.reddit.com/r/CBD/comments/jiwl74/oil_vs_gummies/

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Can I break open capsules and use sublingually?

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Visit our community site for vetted suppliers at http://theCBD.place. It’s time that this subject was given more internet exposure. We are here to discuss topics related to medical marijuana and our experiences using CBD. Please do not assume that anyone here is a medical professional.

Source: https://www.reddit.com/r/CBD/comments/jivcg8/can_i_break_open_capsules_and_use_sublingually/

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