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Development of a simple and sensitive liquid chromatography triple quadrupole mass spectrometry (LC-MS/MS) method for the determination of cannabidiol (CBD), Δ(9)-tetrahydrocannabinol (THC) and its metabolites in rat whole blood after oral administration of a single high dose of CBD

The investigation of the possible conversion of cannabidiol (CBD) into Δ⁹-tetrahydrocannabinol (THC) in vivo after oral administration of CBD is reported herein since recent publications suggested a rapid conversion in simulated gastric fluid. To this end, single high dose of CBD (50mg/kg) was administered orally to rats and their blood was collected after 3 and 6h. A highly sensitive and selective LC-MS/MS method was developed and fully validated in compliance with the Scientific Working Group…

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. 2018 Feb 20;150:25-32.

doi: 10.1016/j.jpba.2017.11.054. Epub 2017 Nov 28.

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Federica Palazzoli et al. J Pharm Biomed Anal. .

Abstract

The investigation of the possible conversion of cannabidiol (CBD) into Δ9-tetrahydrocannabinol (THC) in vivo after oral administration of CBD is reported herein since recent publications suggested a rapid conversion in simulated gastric fluid. To this end, single high dose of CBD (50mg/kg) was administered orally to rats and their blood was collected after 3 and 6h. A highly sensitive and selective LC-MS/MS method was developed and fully validated in compliance with the Scientific Working Group of Forensic Toxicology (SWGTOX) standard practices for method validation in forensic toxicology. This method also involved the optimization of cannabinoids and their metabolites extraction in order to remove co-eluting phospholipids and increase the sensitivity of the MS detection. Neither THC nor its metabolites were detected in rat whole blood after 3 or 6h from CBD administration. After oral administration, the amount of CBD dissolved in olive oil was higher than that absorbed from an ethanolic solution. This could be explained by the protection of lipid excipients towards CBD from acidic gastric juice.

Keywords: Cannabidiol; Cannabinoids extraction; Liquid chromatography; Mass spectrometry; Δ(9)-Tetrahydrocannabinol.

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Source: https://pubmed.ncbi.nlm.nih.gov/29202305/?utm_source=no_user_agent&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1TUbR4JfRTv1rbahLd1HaiF0w5ryO5ocWB3_xk3Prq78L0AEhI&fc=20200804222312&ff=20200817013501&v=2.11.5

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My Mom Is In A Lot Of Pain. So Much Nerve Damage They Had To Remove 3 Disks In Her Neck. Assistance On CBD And Med Interactions, Please!

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My mom is taking Januvia, Glipizide, Lantus Injections, and Trulicity Injections. I really think gummies with CBD:THC 100:1 would really help her. Unfortunately, I have heard that diabetes medications might interfere with CBD and vice versa. Does anyone have any experience with interactions with these medications? None of her prescriptions have the grapefruit warning on the labels.

Edited to make corrections.

Source: https://www.reddit.com/r/CBD/comments/l23ooi/my_mom_is_in_a_lot_of_pain_so_much_nerve_damage/

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MP Trae el Flow del Año 3000: Su Papel en Red Bull, Su Anhelo de FMS y su Tema con Kódigo

ElPlanteo.com entrevista al freestyler y músico MP, también conocido como “El Juvenil”. Nacido en 1999 su nombre viene, según dice, de que se ve muy joven en escena.

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MP Trae el Flow del Año 3000: Su Papel en Red Bull, Su Anhelo de FMS y su Tema con Kódigo | High Times





























Source: https://hightimes.com/espanol/mp-trae-el-flow-del-ano-3000-su-papel-en-red-bull-su-anhelo-de-fms-y-su-tema-con-kodigo/

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Bert Kreischer on Comedy Shows During COVID And Hanging Out With Snoop Dogg

Comedian Bert Kreisher is a “go big or go home” type of man.

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Bert Kreischer on Comedy Shows During COVID And Hanging Out With Snoop Dogg | High Times






























Source: https://hightimes.com/celebrities/bert-kreischer-comedy-shows-during-covid-hanging-out-with-snoop-dogg/

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