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Determination of 11 Cannabinoids in Biomass and Extracts of Different Varieties of Cannabis Using High-Performance Liquid Chromatography

An HPLC single-laboratory validation was performed for the detection and quantification of the 11 major cannabinoids in most cannabis varieties, namely, cannabidiolic acid (CBDA), cannabigerolic acid (CBGA), cannabigerol (CBG), cannabidiol (CBD), tetrahydrocannabivarin (THCV), cannabinol (CBN), Δ9-trans-tetrahydrocannabinol (Δ9-THC), Δ8-trans-tetrahydrocannabinol (Δ8-THC), cannabicyclol (CBL), cannabichromene (CBC), and Δ9-tetrahydrocannabinolic acid-A (THCAA). The analysis was carried out on…

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An HPLC single-laboratory validation was performed for the detection and quantification of the 11 major cannabinoids in most cannabis varieties, namely, cannabidiolic acid (CBDA), cannabigerolic acid (CBGA), cannabigerol (CBG), cannabidiol (CBD), tetrahydrocannabivarin (THCV), cannabinol (CBN), Δ9-trans-tetrahydrocannabinol (Δ9-THC), Δ8-trans-tetrahydrocannabinol (Δ8-THC), cannabicyclol (CBL), cannabichromene (CBC), and Δ9-tetrahydrocannabinolic acid-A (THCAA). The analysis was carried out on the biomass and extracts of these varieties. Methanol-chloroform (9:1, v/v) was used for extraction, 4-androstene-3,17-dione was used as the internal standard, and separation was achieved in 22.2 min on a C18 column using a two- step gradient elution. The method was validated for the 11 cannabinoids. The concentration-response relationship of the method indicated a linear relationship between the concentration and peak area with r2 values of >0.99 for all 11 cannabinoids. Method accuracy was determined through a spike study, and recovery ranged from 89.7 to 105.5% with an RSD of 0.19 to 6.32% for CBDA, CBD, THCV, CBN, Δ9-THC, CBL, CBC, and THCAA; recovery was 84.7, 84.2, and 67.7% for the minor constituents, CBGA, CBG, and Δ8-THC, respectively, with an RSD of 2.58 to 4.96%. The validated method is simple, sensitive, and reproducible and is therefore suitable for the detection and quantification of these cannabinoids in different types of cannabis plant materials.

Source: https://pubmed.ncbi.nlm.nih.gov/26651563/?utm_source=no_user_agent&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1XAOX_jnjTyCUo06Xbq0lslwi760CIxcWfQx0n9x5BPH5yV1sN&fc=20200804212910&ff=20200812014057&v=2.11.5

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Growth of Cannabidiol Oil (CBD Oil) Market Size Report Till 2025

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The Global Cannabidiol Oil (CBD Oil) Market covers explicit information regarding the development rate, market estimates, drivers, limitations, future … Source:

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Hemp Seed Market Explosive Factors of Revenue by Major Manufacturers |Hempco Inc, Ecofibre …

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Hemp Seed Market research report is the high quality report having in-depth market research studies. This market report provides best solutions for … Source:

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CBD Oil Market 2021 Global Industry Size, Reviews, Segments, Revenue, and Forecast to 2027

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Fort Collins, Colorado: The most recently published CBD Oil Market report provides a critical assessment of key growth dynamics, emerging pathways, … Source:

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