Connect with us

Heartland

Cannabidiolic acid dampens the expression of cyclooxygenase-2 in MDA-MB-231 breast cancer cells: Possible implication of the peroxisome proliferator-activated receptor β/δ abrogation

A growing body of experimental evidence strongly suggests that cannabidiolic acid (CBDA), a major component of the fiber-type cannabis plant, exerts a variety of biological activities. We have reported that CBDA can abrogate cyclooxygenase-2 (COX-2) expression and its enzymatic activity. It is established that aberrant expression of COX-2 correlates with the degree of malignancy in breast cancer. Although the reduction of COX-2 expression by CBDA offers an attractive medicinal application, the…

Republished by Plato

Published

on

A growing body of experimental evidence strongly suggests that cannabidiolic acid (CBDA), a major component of the fiber-type cannabis plant, exerts a variety of biological activities. We have reported that CBDA can abrogate cyclooxygenase-2 (COX-2) expression and its enzymatic activity. It is established that aberrant expression of COX-2 correlates with the degree of malignancy in breast cancer. Although the reduction of COX-2 expression by CBDA offers an attractive medicinal application, the molecular mechanisms underlying these effects have not fully been established. It has been reported that COX-2 expression is positively controlled by peroxisome proliferator-activated receptor β/δ (PPARβ/δ) in some cancerous cells, although there is “no” modulatory element for PPARβ/δ on the COX-2 promoter. No previous studies have examined whether an interaction between PPARβ/δ-mediated signaling and COX-2 expression exists in MDA-MB-231 cells. We confirmed, for the first time, that COX-2 expression is positively modulated by PPARβ/δ-mediated signaling in MDA-MB-231 cells. CBDA inhibits PPARβ/δ-mediated transcriptional activation stimulated by the PPARβ/δ-specific agonist, GW501516. Furthermore, the disappearance of cellular actin stress fibers, a hallmark of PPARβ/δ and COX-2 pathway activation, as evoked by the GW501516, was effectively reversed by CBDA. Activator protein-1 (AP-1)-driven transcriptional activity directly involved in the regulation of COX-2 was abrogated by the PPARβ/δ-specific inverse agonists (GSK0660/ST-247). Thus, it is implicated that there is positive interaction between PPARβ/δ and AP-1 in regulation of COX-2. These data support the concept that CBDA is a functional down-regulator of COX-2 through the abrogation of PPARβ/δ-related signaling, at least in part, in MDA-MB-231 cells.

Keywords: Breast cancer; COX-2; Cannabidiolic acid; MDA-MB-231; PPARβ/δ; Stress fiber.

Source: https://pubmed.ncbi.nlm.nih.gov/32238697/?utm_source=no_user_agent&utm_medium=rss&utm_campaign=pubmed-2&utm_content=18gXB4q-CV5o0kQDSCt3HqwNcsXbn1PxqekJlWJaIbT8zAG16G&fc=20200804213200&ff=20200811034631&v=2.11.5

Heartland

Republished by Plato

Published

on

Source:

Continue Reading

Heartland

Republished by Plato

Published

on

Source:

Continue Reading

Heartland

Republished by Plato

Published

on

Source:

Continue Reading
Heartland2 days ago

Legal Status of CBD in Indiana?

Heartland5 days ago

Fly with Cannabis – Which Countries Let You Do It

CBD4 days ago

EU Beat France, CBD Legal Throughout EU

Heartland4 days ago

Suspitcions on a CBD shop in my city

Heartland2 days ago

Germany Rejected Its Recreational Cannabis Bill

Heartland2 days ago

Germany Rejected Its Recreational Cannabis Bill

Heartland2 days ago

Germany Rejected Its Recreational Cannabis Bill

Heartland4 days ago

Anyone tried or heard of “plant love naturals”?

Heartland23 hours ago

Lebanon Legalized Medical Cannabis, 1st in Arab World

Heartland23 hours ago

Lebanon Legalized Medical Cannabis, 1st in Arab World

Heartland23 hours ago

Lebanon Legalized Medical Cannabis, 1st in Arab World

Heartland3 days ago

Grado Labs Review – The Hemp Limited Edition Headphone

Heartland5 days ago

European Court of Justice Rules that CBD is not a narcotic

Metal4 days ago

Turkish metal producers’ foreign sales prices up 5.85% in Oct from Sept

Heartland4 days ago

New Jersey lawmakers take another major step toward launching legal cannabis

Material4 days ago

Multifunctional Flexible and Stretchable Graphite-Silicone Rubber Composites

Material4 days ago

Morphological, structural, thermal properties of a native starch obtained from babassu mesocarp for food packaging application

Material2 days ago

Injection of H2-rich carbonaceous materials into the Blast furnace: devolatilization, gasification and combustion characteristics and effect of increased H2-H2O on iron ore pellets reducibility

Heartland5 days ago

Huge win for European CBD Industry

Heartland4 days ago

10mg capsules

Heartland3 days ago

Did Alex Trebek Run a CBD Oil Business?

Manufacturing5 days ago

Remote Camera Doorbell And Smart Lock With Raspberry Pi

Uncategorized3 days ago

European Court of Justice Rules CBD not a Narcotic

Heartland3 days ago

In the weeds: Greg Atkinson navigates the changing markets for hemp products

Material2 days ago

Strain-induced precipitation in Ti microalloyed steel by two-stage controlled rolling process

Heartland4 days ago

Best Short Strains

Heartland4 days ago

Heartland4 days ago

Heartland4 days ago

Heartland4 days ago

The Daily Hit: November 19th, 2020

Trending

A Cloud Nine Capital Entity Copyright © 2020 – All Rights Reserved Proudly Made in America