Connect with us

Heartland

Cannabidiol modulates serotonergic transmission and reverses both allodynia and anxiety-like behavior in a model of neuropathic pain

Clinical studies indicate that cannabidiol (CBD), the primary nonaddictive component of cannabis that interacts with the serotonin (5-HT)1A receptor, may possess analgesic and anxiolytic effects. However, its effects on 5-HT neuronal activity, as well as its impact on models of neuropathic pain are unknown. First, using in vivo single-unit extracellular recordings in rats, we demonstrated that acute intravenous (i.v.) increasing doses of CBD (0.1-1.0 mg/kg) decreased the firing rate of 5-HT…

Republished by Plato

Published

on

Figure 3.

TRPV1 antagonism blocked the antinociceptive, but not the anxiolytic effects, of repeated CBD administration in SNI rats. (A) Scheme illustrating the timing of SNI induction, behavioral and electrophysiological experiments. (B) Mechanical hypersensitivity was measured with von Frey filaments at day 15 (D0), 19 (D5), and 23 (D7) after SNI surgery. Spared nerve injury rats treated with veh (n = 9), CBD alone (5 mg/kg/day, subcutaneously [s.c.], for 7 days, n = 9), or in combination with CPZ (10 mg/kg/day, s.c., for 7 days, 10 minutes before CBD, n = 8) and CPZ alone (10 mg/kg/day, s.c., for 7 days, n = 7) were tested. Each point of the line represents mean ± SEM expressed as mean of 50% paw withdrawal threshold (g). Two-way ANOVA for repeated measures followed by Bonferroni post hoc comparisons. ***P < 0.001 vs SNI/veh; ###P < 0.001 vs SNI/CPZ + CBD at D7. (C) Horizontal movement traces in the OFT of SNI rats treated with veh, CBD alone (5 mg/kg/day, s.c., for 7 days), or in combination with CPZ (10 mg/kg/day, s.c., for 7 days, 10 minutes before CBD) and CPZ alone (10 mg/kg/day, s.c., for 7 days). (D-F) Distance travelled (cm) (D), time spent (s) in the central area (E), and number of entries in the central area (F) of the OFT. Spared nerve injury rats treated with veh (n = 9), CBD alone (5 mg/kg/day, s.c., for 7 days, n = 9), or in combination with CPZ (10 mg/kg/day, s.c., for 7 days, 10 minutes before CBD, n = 8) and CPZ alone (2 mg/kg, s.c., for 7 days, n = 7) were tested. Each bar represents mean ± SEM. In D-F, each point represents data from an individual rat. One-way ANOVA followed by Bonferroni post hoc comparisons. *P < 0.05 and **P < 0.01 vs SNI/veh. (G) Percentage of time (%) spent in the open arms in the EPMT. Each bar represents mean ± SEM. (H) Horizontal movement traces in the EPMT of SNI rats treated with veh (n = 9), CBD alone (5 mg/kg/day, s.c., for 7 days, n = 9), or in combination with CPZ (10 mg/kg/day, s.c., for 7 days, 10 minutes before CBD, n = 8) and CPZ alone (10 mg/kg/day, s.c., n = 7) were tested. (I and J) Latency to feed (s) in the novel (I) and familiar (J) environments in the NSFT. Each symbol represents mean ± SEM. In G, each point represents data from an individual rat. One-way ANOVA followed by Bonferroni post hoc comparisons. *P < 0.05, **P < 0.01, and ***P < 0.001 vs SNI/veh. ANOVA, analysis of variance; CBD, cannabidiol; CPZ, capsazepine; EPMT, elevated plus maze test; N.S., not significant; NSFT, novelty-suppressed feeding test; OFT, open-field test; SNI, spared nerve injury.

Source: https://pubmed.ncbi.nlm.nih.gov/30157131/?utm_source=no_user_agent&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1LgINZmdEb7hTii13TOa5DGOR2cvkVQ2sw4N7-xSaEiod6ucE1&fc=20200804221722&ff=20200815202741&v=2.11.5

Heartland

Republished by Plato

Published

on

Source:

Continue Reading

Heartland

Republished by Plato

Published

on

Source:

Continue Reading

Heartland

Republished by Plato

Published

on

Source:

Continue Reading
Heartland1 day ago

CBD Vape Oil Market 2021: Global Trends, Business Overview, Challenges, Opportunities …

Heartland4 days ago

Uncategorized5 days ago

What CBD Oil is Right For Me?

Heartland4 days ago

CBD Oil for Beginners

Heartland4 days ago

Heartland3 days ago

Heartland5 days ago

Registration opens for consumable hemp manufacturers and retailers

Heartland5 days ago

CBD Skincare Products Are Popping Up Everywhere — But Do They Actually Work?

Heartland4 days ago

CBP Can't Block Parts Imports, Hemp Machinery Co. Says

Heartland3 days ago

8 of the best cannabis gummies to try right now

Heartland3 days ago

National Hemp Association Awards First Recipients of Social Equity Conscious Business (SECB …

Heartland2 days ago

Industrial Hemp in Automotive Market Estimated Forecast Analysis 2021-2030

Material2 days ago

Microstructure, characterization of interfacial phases and mechanical properties of high Nb–TiAl/Al2O3 joints brazed by novel Nb particle-reinforced Ag–Cu filler alloy

Heartland2 days ago

Drew Carey CBD – How Does It Work For Body Pain

Heartland5 days ago

Interactions between CBD and hormonal contraception

Metal5 days ago

Pakistani CRC and HDG producers raise prices on higher input costs

Heartland5 days ago

12 Ways CBD products can Improve your Health and Wellbeing – 2021 Review

Heartland4 days ago

Sunnyvale Labs CBD Gummies — Instant Pain Relief Hemp Gummies!

Heartland4 days ago

Heartland3 days ago

Heartland5 days ago

Material5 days ago

IMC microstructure modification and mechanical reinforcement of Sn–Ag–Cu/Cu microelectronic joints through an advanced surface finish technique

Heartland4 days ago

Hemp Is Creating an EcoRevolution

Heartland4 days ago

CBD to combat physcoactive properties in THC

Heartland4 days ago

Heartland5 days ago

Former Eaze CEO Pleads Guilty In Bank Fraud Case

Heartland5 days ago

Material5 days ago

A new approach of predicting dynamic recrystallization using directly a flow stress model and its application to medium Mn steel

Heartland4 days ago

Acreage Holdings to Sell Florida Cannabis Operations to Red White & Bloom for $60 Million

Uncategorized4 days ago

The Daily Hit: The February 25th, 2021

Trending

A Cloud Nine Capital Entity Copyright © 2020 – All Rights Reserved Proudly Made in America